MAP2 Rabbit Polyclonal Antibody (HA500177)
Catalog# HA500177
Rabbit Polyclonal to MAP2
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WB
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IF-Cell
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IHC-P
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mIHC
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Human
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Mouse
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Rat
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 200 kDa
Observed band size: 300/70 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500177) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
MAP2 Rabbit Polyclonal Antibody (HA500177)
Immunogen
Synthetic peptide within human map2 aa 1760-1810.
Host
Rabbit
Positive Control
Mouse brain tissue lysates, rat brain tissue lysates, SH-SY5Y, human brain tissue, mouse brain tissue, rat brain tissue, human prostate carcinoma tissue, mouse hippocampus tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:50-1:100
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IHC-P
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1:200-1:1,000
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mIHC
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1:1,000-1:5,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 200 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
MAP2
SYNONYMS
DKFZp686I2148 antibody;MAP 2 antibody;MAP dendrite specific antibody;MAP-2 antibody;MAP2 antibody;MAP2A antibody;MAP2B antibody;MAP2C antibody;Microtubule associated protein 2 antibody;Microtubule-associated protein 2 antibody;MTAP2_HUMAN antibody
SUBCELLULAR LOCATION
Cytoskeleton, dendrite.
FUNCTION
This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described.
CITATIONS
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Yue Liu , Huasong Zhang , Cong Fan , Feiyi Liu , Shaoying Li , Juanjuan Li , Huiying Zhao , Xianhai Zeng
Potential role of Bcl2 in lipid metabolism and synaptic dysfunction of age-related hearing loss
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WB
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Rat
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Citation
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Ni Yinhua,et al
Hydrolyzed Chicken Meat Extract and Its Bioactive Cyclopeptides Protect Neural Function by Attenuating Inflammation and Apoptosis via PI3K/AKT and AMPK Pathways
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IF
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Mouse
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Citation
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Wanshun Wang,et al
Microenvironment-responsive injectable hydrogel for neuro-vascularized bone regeneration
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IF
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rat
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Citation
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