Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [JF0953] (ET1702-04)
Catalog# ET1702-04
Rabbit Monoclonal to Mannose Receptor(CD206)
-
WB
-
ICC/IF
-
Human
-
Mouse
Lane 1: Wild-type HepG2 whole cell lysate (10 µg).
Lane 2: CD206 knockdown HepG2 whole cell lysate (10 µg).
Lane 3: CD206 knockdown HepG2 whole cell lysate (10 µg).
ET1702-04 was shown to specifically react with CD206 in wild-type Hela cells. Weakened bands were observed when CD206 knockdown samples were tested. Wild-type and CD206 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1702-04, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Mannose Receptor(CD206) Recombinant Rabbit Monoclonal Antibody [JF0953] (ET1702-04)
Immunogen
Synthetic peptide within human cd206 aa 1,407-1,456 / 1,456.
Host
Rabbit
Positive Control
Human lung tissue lysate, HepG2 cell lysate, mouse lung tissue lysate, D3 cell lysate, 293T cell lysate, MCF-7 cell lysate, Hela, MCF-7, HepG2.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF0953
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
ICC/IF
-
1:100-1:500
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
166 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Mannose Receptor(CD206)
SYNONYMS
bA541I19.1 antibody;C type lectin domain family 13 member D antibody;C-type lectin domain family 13 member D antibody;CD 206 antibody;CD206 antibody;CD206 antigen antibody;CLEC13D antibody;CLEC13DL antibody;Macrophage mannose receptor 1 antibody;Macrophage mannose receptor 1 like protein 1 antibody;Macrophage mannose receptor antibody;Mannose receptor C type 1 antibody;Mannose receptor C type 1 like 1 antibody;MMR antibody;MRC 1 antibody;MRC1 antibody;MRC1_HUMAN antibody;MRC1L1 antibody;OTTHUMP00000045206 antibody
SUBCELLULAR LOCATION
Endosome membrane, Cell membrane.
FUNCTION
CD206, also known as macrophage mannose receptor type C (MMR or MRC1), is a type I membrane receptor protein. It is an phagocytic and endocytic receptor that can recognize carbohydrate ligands in target molecules. The extracellular portion of the protein includes eight C-type carbohydrate recognition domains (CRD) which are clustered together to achieve higher affinity binding to saccharides. CD206 is found on macrophages and on endothelial cells of the liver and is the only known example of a C-type lectin that contains multiple C-type CRDs. CD206 mediates the endocytosis of glycoproteins by macrophages and binds high-mannose structures on the surface of potentially pathogenic viruses, fungi and bacteria enabling them to be neutralized by phagocytic engulfment. During inflammation, CD206 is crucial for rapid clearance of several mannose-bearing serum glycoproteins but does not regulate the initiation of inflammation. CD206 is primarily expressed in mature tissue macrophages and immature dendritic cells, as well as hepatic and lymphatic endothelial cells, retinal pigmental epithelium (RPE) and mesangial cells.
CITATIONS
-
Lu, X., Yao, J., Li, C., Cui, L., Liu, Y., Liu,...
Shexiang Tongxin Dropping Pills Promote Macrophage Polarization-Induced Angiogenesis Against Coronary Microvascular Dysfunction via PI3K/Akt/mTORC1 Pathway
-
WB
-
Rat
-
Citation
-
Popup
Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.
Age verification
By clicking enter you are verifying that you are old enough to consume alcohol.