PRODUCT CODE: ER1802-93

mAID-tag Rabbit Polyclonal Antibody (ER1802-93)

Applications

  • WB

REACTIVITY

Western blot analysis of Mini-AID-tag on fusion protein using anti-Mini-AID-tag antibody at 1/5,000 dilution.
  • Western blot analysis of Mini-AID-tag on fusion protein using anti-Mini-AID-tag antibody at 1/5,000 dilution.
Western blot analysis of Mini-AID-tag on fusion protein using anti-Mini-AID-tag antibody at 1/5,000 dilution.

Applications

  • WB

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

mAID-tag Rabbit Polyclonal Antibody (ER1802-93)

Immunogen

Synthetic peptide immune sequence is pkdpakppakaqvvgwpc.

Host

Rabbit

Positive Control

Mini-AID-tagged fusion protein.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

TARGET

PROTEIN NAME

mAID-tag

FUNCTION

Auxin-inducible degron (AID) technology allows rapid depletion of proteins in animal cells and fungi. Auxin includes chemicals such as IAA (indole-3-aceticacid) and NAA (1-naphtalene acetic acid) and includes inhibitors in plants. For the degradation, and SCF ubiquitin ligase complex containing the TIR1 protein is activated via the association of auxin with TIR1. Because the core SCF components are conserved in all eukaryotic cells, it is possible to transplant the degradation pathway to yeast and mammalian cells by expressing TIR1. In those cells expressing TIR1, a protein fused with degron (AID degron) derived from AUX/IAA can be rapidly degraded in the presence of auxin. Anti-mini-AID tag can detected both mini-AID-tag and full-length AID-tag.