Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of MAD3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-76, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: SH-SY5Y cell lysate
Lane 2: Hela cell lysate
bHLHc13 antibody;Class C basic helix-loop-helix protein 13 antibody;FLJ35523 antibody;Likely ortholog of mouse Max dimerization protein 3 antibody;MAD3_HUMAN antibody;MAX dimerization protein 3 antibody;Max dimerizer 3 antibody;Max-associated protein 3 antibody;Max-interacting transcriptional repressor MAD3 antibody;MGC2383 antibody;mxd3 antibody;Myx antibody;OTTHUMP00000223596 antibody;OTTHUMP00000223598 antibody
MXD3 is a member of the MXD family of basic-helix-loop-helix transcription factors that form heterodimers with the cofactor MAX, and were originally proposed as functional antagonists of MYC proteins in the MYC/MAX/MAD transcriptional network. While some MXD proteins are MYC antagonists by acting as transcriptional repressors to promote cell differentiation, MXD3 is an atypical member that plays a role in proliferation rather than differentiation. MXD3 promotes granule neuron precursor (GNP) proliferation through a Sonic Hedgehog-mediated pathway, it is expressed in a mouse model of medulloblastoma, and its knock-down (KD) reduced proliferation of the human medulloblastoma cell line DAOY.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.