PRODUCT CODE: ET1706-38

Mad2L2 Recombinant Rabbit Monoclonal Antibody [JU99-23] (ET1706-38)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Mad2L2 on SiHa cell using anti-Mad2L2 antibody at 1/500 dilution.
  • Western blot analysis of Mad2L2 on SiHa cell using anti-Mad2L2 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Mad2L2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with Mad2L2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Mad2L2 on SiHa cell using anti-Mad2L2 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Mad2L2 Recombinant Rabbit Monoclonal Antibody [JU99-23] (ET1706-38)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SiHa, rat lung tissue, mouse colon tissue, human tonsil tissue, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU99-23

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

24 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

Mad2L2

SYNONYMS

Homolog of REV7 S cerevisiae antibody; hREV7 antibody; MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody; MAD2 homolog antibody; MAD2 like 2 antibody; MAD2 mitotic arrest deficient like 2 antibody; MAD2-like protein 2 antibody; MAD2B antibody; Mad2l2 antibody; MD2L2_HUMAN antibody; Mitotic Arrest Deficient 2 L2 antibody; Mitotic arrest deficient 2-like protein 2 antibody; Mitotic arrest deficient homolog like 2 antibody; Mitotic arrest deficient like 2 (yeast) antibody; Mitotic arrest deficient yeast homolog antibody; Mitotic spindle assembly checkpoint protein MAD2B antibody; Polymerase (DNA directed) zeta 2 accessory subunit antibody; POLZ2 antibody; REV 7 antibody; REV7 antibody; REV7 homolog antibody; Weakly similar to Mitotic MAD2 protein (S cerevisiae) antibody

TISSUE SPECIFICITY

Ubiquitously expressed.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD2 (for mitotic arrest-deficient) is a component of the mitotic spindle checkpoint. Cells with mutated MAD2 do not undergo mitotic arrest in response to incorrect spindle fiber assembly, which results in missegregation and eventual cell death. A breast carcinoma cell line with reduced MAD2 expression, T-47D, was shown to complete mitosis in the presence of Nocodazole, an inhibitor of mitotic spindle assembly. MAD2 is localized to unattached kinetochores during pro-metaphase and disassociates upon spindle fiber attachment, indicating that MAD2 regulates kinetochore binding to the spindle fibers. Human MAD2 has also been shown to associate with Insulin receptor (IR), but not IGF-IR, implicating MAD2 as a mediator for IR-specific signaling. MAD2B, a MAD2 homolog, is required for the execution of the mitotic checkpoint monitoring the kinetochore-spindle attachment process and, if the process is not complete, MAD2B delays the onset of anaphase.