PRODUCT CODE: ET1602-5

M6PR Recombinant Rabbit Monoclonal Antibody [SR45-09] (ET1602-5)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of M6PR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-5, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SiHa cell lysate<br />
Lane 2: human skin tissue lysate
  • Western blot analysis of M6PR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-5, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SiHa cell lysate<br />
Lane 2: human skin tissue lysate
  • ICC staining of M6PR in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of M6PR in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of M6PR in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1602-5, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-M6PR antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1602-5, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of M6PR was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1602-5, 1/50) (blue). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; red).
Western blot analysis of M6PR on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1602-5, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: human skin tissue lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

M6PR Recombinant Rabbit Monoclonal Antibody [SR45-09] (ET1602-5)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SiHa cell lysate, human skin tissue lysate, Hela, MCF-7, HepG2, human tonsil tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SR45-09

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

274 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:10,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

M6PR

SYNONYMS

300 kDa mannose 6 phosphate receptor antibody; 300 kDa mannose 6-phosphate receptor antibody; Cation independent mannose 6 phosphate receptor antibody; Cation-independent mannose-6-phosphate receptor antibody; CD222 antibody; CD222 antigen antibody; CI Man 6 P receptor antibody; CI Man-6-P receptor antibody; CI MPR antibody; CI-M6PR antibody; CI-MPR antibody; CIMPR antibody; IGF 2 receptor antibody; IGF 2R antibody; IGF II receptor antibody; IGF-II receptor antibody; IGF2 receptor antibody; Igf2r antibody; Insulin like growth factor 2 receptor antibody; Insulin like growth factor II receptor antibody; Insulin-like growth factor 2 receptor antibody; Insulin-like growth factor II receptor antibody; M6P R antibody; M6P/IGF2 receptor antibody; M6P/IGF2R antibody; M6PR antibody; mannose 6 phosphate receptor antibody; mannose 6 phosphate receptor, cation independent antibody; MPR 300 antibody; MPR300 antibody; MPRI antibody; MPRI_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the MRL1/IGF2R family.

SUBCELLULAR LOCATION

Golgi apparatus membrane, Endosome membrane.

FUNCTION

The mannose 6-phosphate/insulin-like growth factor II receptor, IGF-IIR (also designated M6P/IGF2R), is a ubiquitously expressed integral glycoprotein. By binding glycoproteins through two of its extracytoplasmic domains, IGF-IIR mediates the activation of TGF∫1 (a growth inhibitor), the degradation of IGF-II and the transport of lysosomal enzymes. Subsequently, IGF-IIR can form oligomeric complexes, which increase the affinity of IGF-IIR for lysosomal enzymes. Unlike IGF-IR, IGF-IIR does not potentiate the signaling of IGF-I or IGF-II, which have mitogenic, cell survival and insulin-like effects. Therefore, IGF-IIR is characterized as a tumor suppressor. Furthermore, the IGF-IIR gene is located on chromosome 6q26, which is commonly mutated or deleted in several human cancers.