Western blot analysis of LYRIC on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-60, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF-7 cell lysate
Lane 2: Jurkat cell lysate
3D3 antibody;3D3/LYRIC antibody;AEG 1 antibody;AEG-1 antibody;AEG1 antibody;Astrocyte elevated gene 1 antibody;Astrocyte elevated gene-1 protein antibody;LYRIC antibody;LYRIC/3D3 antibody;LYRIC_HUMAN antibody;Lysine rich CEACAM1 associated protein antibody;Lysine rich CEACAM1 co isolated protein antibody;Lysine-rich CEACAM1 co-isolated protein antibody;Metadherin antibody;Metastasis adhesion protein antibody;MTDH antibody;Protein LYRIC antibody
Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.
Metadherin, also known as protein LYRIC or astrocyte elevated gene-1 protein (AEG-1) is a protein that in humans is encoded by the MTDH gene. MTDH (AEG-1) is involved in HIF-1alpha mediated angiogenesis. MTDH also interacts with SND1 and involved in RNA-induced silencing complex (RISC) and plays very important role in RISC and miRNA functions. MTDH has been shown to interact with spliceosome proteins in the cell nucleus and regulate the process of alternative splicing. MTDH induces an oncogene called Late SV40 factor (LSF/TFCP2) which is involved in thymidylate synthase (TS) induction and DNA biosynthesis synthesis. Late SV40 factor (LSF/TFCP2) enhances angiogenesis by transcriptionally up-regulating matrix metalloproteinase-9 (MMP9). MTDH acts as an oncogene in melanoma, malignant glioma, breast cancer and hepatocellular carcinoma. It is highly expressed in these cancers and helps in progression and development of these cancers. It is induced by c-Myc oncogene and plays very important role in anchorage independent growth of cancer cells. Elevated expression of the metastasis gene metadherin (MTDH), which is overexpressed in more than 40% of breast cancers, is associated with poor clinical outcomes.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.