Lyn Recombinant Rabbit Monoclonal Antibody [JE44-75] (HA721991)
Catalog# HA721991
Rabbit Monoclonal to Lyn
Tested for
-
WB
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of Lyn on different lysates with Rabbit anti-Lyn antibody (HA721991) at 1/1,000 dilution.
Lane 1: Ramos cell lysate (15 µg/Lane)
Lane 2: A431 cell lysate (15 µg/Lane)
Lane 3: Jurkat cell lysate (15 µg/Lane)
Lane 4: THP-1 cell lysate (15 µg/Lane)
Lane 5: Raji cell lysate (15 µg/Lane)
Lane 6: K-562 cell lysate (15 µg/Lane)
Lane 7: U-937 cell lysate (15 µg/Lane)
Lane 8: Mouse lung tissue lysate (30 µg/Lane)
Lane 9: Rat lung tissue lysate (30 µg/Lane)
Predicted band size: 59 kDa
Observed band size: 55 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721991) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Ramos cell lysate (15 µg/Lane)
Lane 2: A431 cell lysate (15 µg/Lane)
Lane 3: Jurkat cell lysate (15 µg/Lane)
Lane 4: THP-1 cell lysate (15 µg/Lane)
Lane 5: Raji cell lysate (15 µg/Lane)
Lane 6: K-562 cell lysate (15 µg/Lane)
Lane 7: U-937 cell lysate (15 µg/Lane)
Lane 8: Mouse lung tissue lysate (30 µg/Lane)
Lane 9: Rat lung tissue lysate (30 µg/Lane)
Predicted band size: 59 kDa
Observed band size: 55 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721991) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.