Ly6c Rabbit Polyclonal Antibody (HA500088)
Catalog# HA500088
Rabbit Polyclonal to Ly6c
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WB
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IHC-P
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FC
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Mouse
Western blot analysis of Ly6c on different lysates with Rabbit anti-Ly6c antibody (HA500088) at 1/1,000 dilution.
Lane 1: Mouse lung tissue lysate
Lane 2: Mouse spleen tissue lysate
Lane 3: Mouse testis tissue lysate (low expression)
Lane 4: Mouse liver tissue lysate (low expression)
Lane 5: Mouse kidney tissue lysate
Lane 6: Mouse heart tissue lysate
Lane 7: Mouse brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500088) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Ly6c Rabbit Polyclonal Antibody (HA500088)
Immunogen
Synthetic peptide within mouse ly6c1 aa 75-100.
Host
Rabbit
Positive Control
Mouse lung tissue lysate, mouse spleen tissue lysate, mouse testis tissue lysate, mouse kidney tissue lysate, mouse heart tissue lysate, mouse brain tissue lysate, mouse lung tissue, mouse brain tissue, mouse spleen tissue, mouse spleen cells.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IHC-P
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1:100-1:500
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 14 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Ly6c
SYNONYMS
Ly 6c antibody;Ly6c protein antibody;Ly6c1 antibody;Ly6c2 antibody;Lymphocyte antigen 6 complex antibody;Lymphocyte antigen 6 complex locus C antibody;Lymphocyte antigen 6C antibody;Lymphocyte antigen 6C1 antibody;Lymphocyte antigen 6C2 antibody;Lymphocyte antigen Ly 6C antibody
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
The gene encoding the mouse alloantigen, Ly-6C, maps to chromosome 15 and encodes a 131 amino acid protein that belongs to the Ly-6 family of glycosyl-phosphatidylinositol (GPI)-linked proteins. Ly-6 family members share amino acid homology throughout a distinctive cysteine rich protein domain that incorporates O-linked carbohydrates. Murine Ly-6 molecules have unique patterns of tissue expression during hematopoiesis from multipotential stem cells to lineage committed precursor cells, and on specific leukocyte subpopulations in the peripheral lymphoid tissues. Ly-6C is predominantly expressed on murine peripheral CD8 T cells. Ly-6C is involved in endothelial adhesion, the killing of target cells by CTLs, inducing TCR-mediated activation of IL-2 and IFN-γ production in CD8 T cells and the homing of CD8 T cells. In addition, Ly-6C may act as a signaling molecule of LFA-1 activation.
CITATIONS
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Garcia-Flores, V., Romero, R., Peyvandipour, A., Galaz, J., Pusod, E., Panaitescu, B., Miller, D., Xu, Y., Tao, L., Liu, Z., Tarca, A. L., Pique-Regi, R., & Gomez-Lopez, N.
A single-cell atlas of murine reproductive tissues during preterm labor
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IF
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Mouse
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Citation
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