Ly6c Rabbit Polyclonal Antibody (HA500087)
Catalog# HA500087
Rabbit Polyclonal to Ly6c
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WB
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IHC-P
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FC
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Mouse
Lane 1: C2C12 cell lysate
Lane 2: Mouse lung tissue lysate
Lane 3: Mouse spleen tissue lysate
Lane 4: Mouse kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 14 kDa
Observed band size: 14 kDa
Exposure time: 1 minute 50 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500087) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Ly6c Rabbit Polyclonal Antibody (HA500087)
Immunogen
Synthetic peptide within mouse ly6c2 aa 45-77.
Host
Rabbit
Positive Control
C2C12 cell lysate, Mouse lung tissue lysate, Mouse spleen tissue lysate, Mouse kidney tissue lysate, mouse heart tissue, mouse spleen cells.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:200
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 14 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Ly6c
SYNONYMS
Ly 6c antibody;Ly6c protein antibody;Ly6c1 antibody;Ly6c2 antibody;Lymphocyte antigen 6 complex antibody;Lymphocyte antigen 6 complex locus C antibody;Lymphocyte antigen 6C antibody;Lymphocyte antigen 6C1 antibody;Lymphocyte antigen 6C2 antibody;Lymphocyte antigen Ly 6C antibody
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
The gene encoding the mouse alloantigen, Ly-6C, maps to chromosome 15 and encodes a 131 amino acid protein that belongs to the Ly-6 family of glycosyl-phosphatidylinositol (GPI)-linked proteins. Ly-6 family members share amino acid homology throughout a distinctive cysteine rich protein domain that incorporates O-linked carbohydrates. Murine Ly-6 molecules have unique patterns of tissue expression during hematopoiesis from multipotential stem cells to lineage committed precursor cells, and on specific leukocyte subpopulations in the peripheral lymphoid tissues. Ly-6C is predominantly expressed on murine peripheral CD8 T cells. Ly-6C is involved in endothelial adhesion, the killing of target cells by CTLs, inducing TCR-mediated activation of IL-2 and IFN-γ production in CD8 T cells and the homing of CD8 T cells. In addition, Ly-6C may act as a signaling molecule of LFA-1 activation.
CITATIONS
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Integrated Bioinformatics Analysis and Experimental Verification of Immune Cell Infiltration and the Related Core Genes in Ulcerative Colitis
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IHC
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Mouse
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Citation
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