Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Lumican on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate
Lane 2: 293T cell lysate
Lane 3: Human skin tissue lysate
Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.
Cornea and other tissues.
Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.
Sulfated on tyrosine residue(s).
Extracellular matrix. Secreted.
Lumican, a member of the small leucine-rich proteoglycans (SLRPs) family of proteins, is a keratan sulfate proteoglycan present in large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. During collagen fibrillogenesis, lumican regulates the assembly of collagens into higher order fibrils, limiting the growth of fibrils in diameter. Lumican is present in the extracellular matrix of human articular cartilage at all ages, with a far greater abundance in the adult. The gene encoding human lumican maps to chromosome 12q21.3-q22.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.