PRODUCT CODE: ET7109-24

Lumican Recombinant Rabbit Monoclonal Antibody [JE11-45] (ET7109-24)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Lumican on different lysates using anti-Lumican antibody at 1/1,000 dilution. <br />
 Positive control: <br />
 Lane 1: Jurkat  <br />
          Lane 2: 293T <br />
 Lane 3: Human skin
  • Western blot analysis of Lumican on different lysates using anti-Lumican antibody at 1/1,000 dilution. <br />
 Positive control: <br />
 Lane 1: Jurkat  <br />
          Lane 2: 293T <br />
 Lane 3: Human skin
  • ICC staining Lumican in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Lumican in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Lumican in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Lumican antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Lumican antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with Lumican antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).  Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Lumican on different lysates using anti-Lumican antibody at 1/1,000 dilution.
Positive control:
Lane 1: Jurkat
Lane 2: 293T
Lane 3: Human skin

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Lumican Recombinant Rabbit Monoclonal Antibody [JE11-45] (ET7109-24)

Immunogen

Recombinant protein within human lumican aa 50-250.

Host

Rabbit

Positive Control

Jurkat, 293T, Human skin tissue, MCF-7, MG-63, PANC-1, rat kidney tissue, human skin tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE11-45

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Lumican

SYNONYMS

Keratan sulfate proteoglycan lumican antibody; KSPG lumican antibody; LDC antibody; LUM antibody; LUM_HUMAN antibody; Lumican antibody; Lumican precursor antibody; SLRR2D antibody

SEQUENCE SIMILARITIES

Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class II subfamily.

TISSUE SPECIFICITY

Cornea and other tissues.

DEVELOPMENTAL STAGE

Present in the extracellular matrix of human articular cartilage at all ages, although its abundance is far greater in the adult. In the adult cartilage lumican exists predominantly in a glycoprotein form lacking keratan sulfate, whereas the juvenile form of the molecule is a proteoglycan.

POST-TRANSLATIONAL MODIFICATION

Sulfated on tyrosine residue(s).

SUBCELLULAR LOCATION

Extracellular matrix. Secreted.

FUNCTION

Lumican, a member of the small leucine-rich proteoglycans (SLRPs) family of proteins, is a keratan sulfate proteoglycan present in large quantities in the corneal stroma and in interstitial collagenous matrices of the heart, aorta, skeletal muscle, skin, and intervertebral discs. During collagen fibrillogenesis, lumican regulates the assembly of collagens into higher order fibrils, limiting the growth of fibrils in diameter. Lumican is present in the extracellular matrix of human articular cartilage at all ages, with a far greater abundance in the adult. The gene encoding human lumican maps to chromosome 12q21.3-q22.