Applications
-
WB
-
ICC
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
LSD1 Rabbit Polyclonal Antibody (ER1802-12)
Immunogen
Synthetic peptide within c-terminal human lsd1.
Host
Rabbit
Positive Control
PC-12, SiHa, A431, HepG2, SH-SY-5Y, rat kidney tissue, human colon cancer tissue, human spleen tissue, human kidney tissue, mouse cerebellum tissue, K562.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified
MOLECULAR WEIGHT
110 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1,000
-
ICC
-
1:500-1:2,000
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
LSD1
SYNONYMS
Amine oxidase (flavin containing) domain 2 antibody; Amine oxidase, flavin containing, 2 antibody; AOF2 antibody; BHC110 antibody; BRAF35 HDAC complex protein BHC110 antibody; BRAF35-HDAC complex protein BHC110 antibody; BRAF35/HDAC complex, 110-kD subunit antibody; CPRF antibody; EC1 antibody; FAD binding protein BRAF35 HDAC complex, 110 kDa subunit antibody; Flavin-containing amine oxidase domain-containing protein 2 antibody; KDM 1 antibody; KDM1 antibody; Kdm1a antibody; KDM1A_HUMAN antibody; KIAA0601 antibody; LSD 1 antibody; LSD1 antibody; Lysine (K) specific demethylase 1 antibody; Lysine (K) specific demethylase 1A antibody; Lysine demethylase 1A antibody; Lysine specific histone demethylase 1 antibody; Lysine specific histone demethylase 1A antibody; Lysine-specific demethylase 1 antibody; Lysine-specific demethylase 1A antibody; Lysine-specific histone demethylase 1A antibody
SEQUENCE SIMILARITIES
Belongs to the flavin monoamine oxidase family.
TISSUE SPECIFICITY
Ubiquitously expressed.
POST-TRANSLATIONAL MODIFICATION
Polyubiquitinated by JADE2; which leads to its proteasomal degradation.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Histone methylation regulates chromatin structure and transcription and maintains an epigenetic state of the cell. Histone methylation is dynamically regulated by histone methylases and demethylases. Lysine-specific histone demethylase 1 (LSD1), also designated BHC110, is a flavin-dependent amine oxidase which catalyzes the removal of one or two methyl groups from the methyl-lysine-4 side chain of Histone H3. The LSD1 protein contains a SWIRM domain, a FAD-binding motif and an amine oxidase domain. Association with CoREST, a SANT domain-containing corepressor, positively regulates LSD1. CoREST mediates the demethylation ability of LSD1 and protects it from proteasomal degradation in vivo. PHF21A (also designated BCH80), a PHD domain-containing protein, inhibits activity of LSD1/CoREST mediated de-methylation. The LSD1 protein also co-localizes with the androgen receptor in human prostate tumor cells and in unaffected prostate cells, stimulating androgen-receptor-dependent transcription.