LRP6 Mouse Monoclonal Antibody [A5E7] (HA600065)
Catalog# HA600065
Mouse Monoclonal to LRP6
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WB
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IHC-P
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Human
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 180 kDa
Observed band size: 230 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600065) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
LRP6 Mouse Monoclonal Antibody [A5E7] (HA600065)
Immunogen
Recombinant protein within human lrp6 aa 100-300 / 1613.
Host
Mouse
Positive Control
HeLa cell lysate, HepG2 cell lysate, human colon tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A5E7
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Molecular Weight
Predicted band size: 180 kDa
Isotype
IgG2b
TARGET
UNIPROT #
PROTEIN NAME
LRP6
SYNONYMS
ADCAD2 antibody;C030016K15Rik antibody;Cd antibody;FLJ90062 antibody;FLJ90421 antibody;LDL receptor related protein 6 antibody;Low density lipoprotein receptor related protein 6 antibody;Low-density lipoprotein receptor-related protein 6 antibody;LRP-6 antibody;LRP6 antibody;LRP6_HUMAN antibody;OTTHUMP00000238979 antibody;OTTHUMP00000238980 antibody;OTTHUMP00000238982 antibody;STHAG7 antibody
SUBCELLULAR LOCATION
Endoplasmic reticulum, Cell membrane, Membrane raft.
FUNCTION
Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. Required for posterior patterning of the epiblast during gastrulation.
CITATIONS
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Circulating neutrophils activated by cancer cells and M2 macrophages promote gastric cancer progression during PD-1 antibody-based immunotherapy
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IHC
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Human
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Citation
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