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PRODUCT CODE: ER1706-53

LOX-1 Rabbit Polyclonal Antibody (ER1706-53)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of LOX1 on siHa cell lysate using anti-LOX1 antibody at 1/100 dilution.
  • Western blot analysis of LOX1 on siHa cell lysate using anti-LOX1 antibody at 1/100 dilution.
  • ICC staining LOX1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining LOX1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LOX1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LOX1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-LOX1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of LOVO cells with LOX1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
  • Western blot analysis of LOX1 on human lung tissue lysate using anti-LOX1 antibody at 1/500 dilution.
Western blot analysis of LOX1 on siHa cell lysate using anti-LOX1 antibody at 1/100 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

LOX-1 Rabbit Polyclonal Antibody (ER1706-53)

Immunogen

Recombinant protein within human lox-1 aa 58-273.

Host

Rabbit

Positive Control

siHa, Hela, LOVO, human lung tissue lysate, human kidney tissue, human placenta tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

31 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P78380

PROTEIN NAME

LOX-1

SYNONYMS

C-type lectin domain family 8 member A antibody; CLEC8A antibody; hLOX 1 antibody; hLOX-1 antibody; Lectin like oxidized LDL receptor 1 antibody; Lectin like oxLDL receptor 1 antibody; Lectin type oxidized LDL receptor 1 antibody; Lectin-like oxidized LDL receptor 1 antibody; Lectin-like oxLDL receptor 1 antibody; Lectin-type oxidized LDL receptor 1 antibody; low density lipoprotein oxidized, receptor 1 antibody; LOX-1 antibody; LOXIN antibody; Olr1 antibody; OLR1_HUMAN antibody; Ox LDL receptor 1 antibody; Ox-LDL receptor 1 antibody; Oxidised low density lipoprotein (lectin like) receptor 1 antibody; Oxidized low density lipoprotein receptor 1 antibody; Oxidized low density lipoprotein receptor 1 soluble form antibody; Oxidized low-density lipoprotein receptor 1 antibody; SCARE1 antibody; Scavenger receptor class E, member 1 antibody; SLOX1 antibody; soluble form antibody; SR-EI antibody

TISSUE SPECIFICITY

Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level.

POST-TRANSLATIONAL MODIFICATION

The intrachain disulfide-bonds prevent N-glycosylation at some sites.; N-glycosylated.

SUBCELLULAR LOCATION

Cell membrane. Secreted.

FUNCTION

The oxidized low density lipoprotein (lectin-like) receptor-1, Ox-LDL R-1, is a type II membrane protein that is a member of the C-type lectin family and acts as a cell-surface receptor for oxidized low density lipoprotein (Ox-LDL). Ox-LDL plays a role in early atherosclerosis, which includes the transformation of monocyte-derived macrophages to foam cells in atherosclerotic lesions. The binding of Ox-LDL to Ox-LDL R-1 may also trigger the activation of the NFkB signal transduction pathway. Ox-LDL R-1, also designated scavenger receptor class E, member 1 (SCARE1); lectin-type oxidized LDL receptor 1 (LOX-1); and CLEC8A), is expressed by vascular endothelial cells, smooth muscle cells and macrophages. It is expressed endogenously as a precursor form with N-linked high mannose carbohydrate chains and as a mature form due to further glycosylation. The N-linked glycosylation of Ox-LDL R-1 appears to be necessary for adequate transportation to the cell surface and efficient ligand binding.