Western blot analysis of LOX-1 on MCF-7 cell lysates with Rabbit anti-LOX-1 antibody (ER1706-53) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 50 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1706-53) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Rabbit polyclonal primary
LOX-1 Rabbit Polyclonal Antibody (ER1706-53)
Recombinant protein within human lox-1 aa 17-273 / 273.
MCF-7, Hela, LOVO, human lung tissue lysate, human kidney tissue, human placenta tissue, mouse brain tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
C-type lectin domain family 8 member A antibody;CLEC8A antibody;hLOX 1 antibody;hLOX-1 antibody;Lectin like oxidized LDL receptor 1 antibody;Lectin like oxLDL receptor 1 antibody;Lectin type oxidized LDL receptor 1 antibody;Lectin-like oxidized LDL receptor 1 antibody;Lectin-like oxLDL receptor 1 antibody;Lectin-type oxidized LDL receptor 1 antibody;low density lipoprotein oxidized, receptor 1 antibody;LOX-1 antibody;LOXIN antibody;Olr1 antibody;OLR1_HUMAN antibody;Ox LDL receptor 1 antibody;Ox-LDL receptor 1 antibody;Oxidised low density lipoprotein (lectin like) receptor 1 antibody;Oxidized low density lipoprotein receptor 1 antibody;Oxidized low density lipoprotein receptor 1 soluble form antibody;Oxidized low-density lipoprotein receptor 1 antibody;SCARE1 antibody;Scavenger receptor class E, member 1 antibody;SLOX1 antibody;soluble form antibody;SR-EI antibody
Expressed at high level in endothelial cells and vascular-rich organs such as placenta, lung, liver and brain, aortic intima, bone marrow, spinal cord and substantia nigra. Also expressed at the surface of dendritic cells. Widely expressed at intermediate and low level.
The intrachain disulfide-bonds prevent N-glycosylation at some sites.; N-glycosylated.
Cell membrane. Secreted.
The oxidized low density lipoprotein (lectin-like) receptor-1, Ox-LDL R-1, is a type II membrane protein that is a member of the C-type lectin family and acts as a cell-surface receptor for oxidized low density lipoprotein (Ox-LDL). Ox-LDL plays a role in early atherosclerosis, which includes the transformation of monocyte-derived macrophages to foam cells in atherosclerotic lesions. The binding of Ox-LDL to Ox-LDL R-1 may also trigger the activation of the NFkB signal transduction pathway. Ox-LDL R-1, also designated scavenger receptor class E, member 1 (SCARE1); lectin-type oxidized LDL receptor 1 (LOX-1); and CLEC8A), is expressed by vascular endothelial cells, smooth muscle cells and macrophages. It is expressed endogenously as a precursor form with N-linked high mannose carbohydrate chains and as a mature form due to further glycosylation. The N-linked glycosylation of Ox-LDL R-1 appears to be necessary for adequate transportation to the cell surface and efficient ligand binding.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.