Applications
-
WB
-
IHC-P
REACTIVITY
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
Lnpk Mouse Monoclonal Antibody [11-1] (EM1701-57)
Immunogen
Recombinant protein within mouse lnpk aa 198-425 / 425.
Host
Mouse
Positive Control
Mouse brain tissue, rat spinal cord tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
11-1
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein affinity purified
MOLECULAR WEIGHT
47 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB
-
1:500
-
IHC-P
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Lnpk
SYNONYMS
IDDM20 antibody; Lymphocyte adapter protein antibody; Lymphocyte specific adapter protein antibody; Lymphocyte specific adapter protein Lnk antibody; Lymphocyte-specific adapter protein Lnk antibody; SH2B adapter protein 3 antibody; SH2B adaptor protein 3 antibody; Sh2b3 antibody; SH2B3_HUMAN antibody; Signal transduction protein Lnk antibody
SEQUENCE SIMILARITIES
Belongs to the lunapark family.
TISSUE SPECIFICITY
Expressed in most tissues at basal level, with reinforcement in distal limb buds, genital bud, and in parts of the central nervous system.
DEVELOPMENTAL STAGE
Strongly expressed in both limb and genital buds as is the case for Evx2 and Hoxd genes, in particular Hoxd13. In developing limb buds, it is first seen in 10.5 days old fetuses, in the posterior distal bud, to subsequently extend throughout the distal aspect, in presumptive digits.
POST-TRANSLATIONAL MODIFICATION
Myristoylated; myristoylation is necessary for the endoplasmic reticulum (ER) three-way ER tubular junction formation, but is not required neither for membrane translocation, membrane topology formation, nor for the specific localization to ER membranes.; Phosphorylated. Phosphorylation occurs at Ser-177, Ser-182, Ser-222, Ser-316 and Ser-380 during interphase. Phosphorylation occurs at Ser-114, Ser-153, Ser-194, Thr-211 and Ser-348 during mitosis; these phosphorylations reduce both its homodimerization and the ER three-way tubular junction formation.; Subject to proteasomal degradation following phosphorylation during mitosis.
SUBCELLULAR LOCATION
Endoplasmic reticulum.
FUNCTION
Endoplasmic reticulum (ER)-shaping membrane protein that plays a role in determining ER morphology. Involved in the stabilization of nascent three-way ER tubular junctions within the ER network. May also play a role as a curvature-stabilizing protein within three-way ER tubular junction network. May be involved in limb and central nervous system development.