PRODUCT CODE: EM1701-47

LNP Mouse Monoclonal Antibody [7-3] (EM1701-47)

Applications

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

ICC staining LNP (green) in SHG-44 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining LNP (green) in SHG-44 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat spinal cord tissue using anti-LNP antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-LNP antibody. Counter stained with hematoxylin.
ICC staining LNP (green) in SHG-44 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

LNP Mouse Monoclonal Antibody [7-3] (EM1701-47)

Immunogen

Recombinant protein within mouse lnp aa 198-425 / 425.

Host

Mouse

Positive Control

SHG-44, rat brain tissue, rat spinal cord tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

7-3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

47 kDa

Isotype

IgG1

APPLICATION DILUTION

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

LNP

SYNONYMS

lnp antibody; LNP_HUMAN antibody; Protein lunapark antibody

SEQUENCE SIMILARITIES

Belongs to the lunapark family.

TISSUE SPECIFICITY

Expressed in neural precursor cells, where it is detected at the growth-cone-like structure and branching sites of neurite-like processes.

POST-TRANSLATIONAL MODIFICATION

Myristoylated; myristoylation is necessary for the endoplasmic reticulum (ER) three-way ER tubular junction formation, but is not required neither for membrane translocation, membrane topology formation, nor for the specific localization to ER membranes.; Phosphorylated. Phosphorylation occurs at Ser-177, Ser-182, Ser-217, Ser-227, Ser-321 and Ser-384 during interphase. Phosphorylation occurs at Ser-114, Ser-153, Ser-194, Thr-211 and Ser-353 during mitosis; these phosphorylations reduce both its homodimerization and the ER three-way tubular junction formation.; Subject to proteasomal degradation following phosphorylation during mitosis.

SUBCELLULAR LOCATION

Endoplasmic reticulum.

FUNCTION

Endoplasmic reticulum (ER)-shaping membrane protein that plays a role in determining ER morphology. Involved in the stabilization of nascent three-way ER tubular junctions within the ER network. May also play a role as a curvature-stabilizing protein within three-way ER tubular junction network. May be involved in limb and central nervous system development