Positive control:
Lane 1: Hela
Lane2 :Jurkat
Lane 3: HepG2
Lane 4:Raji
Applications
-
WB
-
ICC
-
IHC-P
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
LMNB2 Rabbit Polyclonal Antibody (ER0403)
Immunogen
Synthetic peptide corresponding to of human lmnb2 aa 571-620 / 620.
Host
Rabbit
Positive Control
Hela, HepG2, Jurkat, Raji, mouse embryonic stem cell human colon carcinoma tissue
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified
MOLECULAR WEIGHT
68 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
ICC
-
1:200
-
IHC-P
-
1:200
TARGET
UNIPROT #
PROTEIN NAME
LMNB2
SYNONYMS
LAMB 2 antibody; LAMB2 antibody; Lamin-B2 antibody; LMN 2 antibody; LMN B2 antibody; LMN2 antibody; LMNB 2 antibody; LMNB2 antibody; LMNB2_HUMAN antibody; MGC2721 antibody; RGD1563803 antibody
SEQUENCE SIMILARITIES
Belongs to the intermediate filament family.
POST-TRANSLATIONAL MODIFICATION
B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.
SUBCELLULAR LOCATION
Nucleus inner membrane
FUNCTION
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD), which is a rare childhood disease characterized by loss of subcutaneous fat from the face and trunk.