PRODUCT CODE: ER0403

LMNB2 Rabbit Polyclonal Antibody (ER0403)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Lamin B2 on different cell lysates using anti-LMNB2 antibody at 1/1000 dilution.<br />
Positive control:    <br />
Lane 1: Hela   <br />
Lane2 :Jurkat  <br />
Lane 3: HepG2  <br />
Lane 4:Raji
  • Western blot analysis of Lamin B2 on different cell lysates using anti-LMNB2 antibody at 1/1000 dilution.<br />
Positive control:    <br />
Lane 1: Hela   <br />
Lane2 :Jurkat  <br />
Lane 3: HepG2  <br />
Lane 4:Raji
  • ICC staining of LMNB2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining of LMNB2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining of LMNB2 in mouse embryonic stem cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-LMNB2 antibody. Counter stained with hematoxylin.
Western blot analysis of Lamin B2 on different cell lysates using anti-LMNB2 antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela
Lane2 :Jurkat
Lane 3: HepG2
Lane 4:Raji

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

LMNB2 Rabbit Polyclonal Antibody (ER0403)

Immunogen

Synthetic peptide corresponding to of human lmnb2 aa 571-620 / 620.

Host

Rabbit

Positive Control

Hela, HepG2, Jurkat, Raji, mouse embryonic stem cell human colon carcinoma tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

68 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

LMNB2

SYNONYMS

LAMB 2 antibody; LAMB2 antibody; Lamin-B2 antibody; LMN 2 antibody; LMN B2 antibody; LMN2 antibody; LMNB 2 antibody; LMNB2 antibody; LMNB2_HUMAN antibody; MGC2721 antibody; RGD1563803 antibody

SEQUENCE SIMILARITIES

Belongs to the intermediate filament family.

POST-TRANSLATIONAL MODIFICATION

B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

SUBCELLULAR LOCATION

Nucleus inner membrane

FUNCTION

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Defects in LMNB2 are a cause of partial acquired lipodystrophy (APLD), which is a rare childhood disease characterized by loss of subcutaneous fat from the face and trunk.