Liver Carboxylesterase 1 Recombinant Rabbit Monoclonal Antibody [JE33-04] (HA721416)
Catalog# HA721416
Rabbit Monoclonal to Liver Carboxylesterase 1
Tested for
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WB
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IHC-P
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IF-Cell
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FC
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Liver Carboxylesterase 1 on different lysates with Rabbit anti-Liver Carboxylesterase 1 antibody (HA721416) at 1/1,000 dilution.
Lane 1: U-937 cell lysate (20 µg/Lane)
Lane 2: Human liver tissue lysate (10 µg/Lane)
Lane 3: Human lung tissue lysate (10 µg/Lane)
Predicted band size: 62.5 kDa
Observed band size: 55 kDa
Exposure time: 16 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: U-937 cell lysate (20 µg/Lane)
Lane 2: Human liver tissue lysate (10 µg/Lane)
Lane 3: Human lung tissue lysate (10 µg/Lane)
Predicted band size: 62.5 kDa
Observed band size: 55 kDa
Exposure time: 16 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721416) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.