Lipocalin-2 Recombinant Rabbit Monoclonal Antibody [JM10-67] (ET1703-39)
Catalog# ET1703-39
Rabbit Monoclonal to Lipocalin-2
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WB
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IF-Tissue
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IHC-P
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Human
Lysates/proteins at 10 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-39) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Lipocalin-2 Recombinant Rabbit Monoclonal Antibody [JM10-67] (ET1703-39)
Immunogen
Synthetic peptide within human lipocalin-2 aa 50-100/198.
Host
Rabbit
Positive Control
A431 cell lysates, human spleen tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM10-67
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Tissue
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1:200
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IHC-P
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 23 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Lipocalin-2
SYNONYMS
24p3 antibody;25 kDa alpha 2 microglobulin related subunit of MMP9 antibody;25 kDa alpha-2-microglobulin-related subunit of MMP-9 antibody;Alpha 2 microglobulin related protein antibody;HGNC:6526 antibody;HNL antibody;Lcn 2 antibody;Lcn2 antibody;Lipocalin-2 antibody;Migration stimulating factor inhibitor antibody;MSFI antibody;Neutrophil gelatinase associated lipocalin antibody;Neutrophil gelatinase associated lipocalin precursor antibody;Neutrophil gelatinase-associated lipocalin antibody;NGAL antibody;NGAL_HUMAN antibody;Oncogene 24p3 antibody;Oncogenic lipocalin 24p3 antibody;p25 antibody;Siderocalin antibody;siderocalin LCN2 antibody;SV40 induced 24P3 protein antibody;Uterocalin antibody
SEQUENCE SIMILARITIES
Belongs to the calycin superfamily. Lipocalin family.
TISSUE SPECIFICITY
Detected in neutrophils (at protein level). Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
In addition to the monomeric mammalian progelatinase, two additional forms of progelatinase have been identified. The shorter of these additional forms is a covalently linked, disulfide-bridged protein that heterodimerizes with a short protein; an α2-Microglobulin-related protein known as neutrophil gelatinase-associated lipocalin (NGAL), which is moderately expressed in breast and lung tissues. NGAL belongs to the lipocalin family and has a high degree of similarity with rat α2-Microglobulin-related protein and mouse protein 24p3. NGAL is able to bind a derivative of the bacterial chemotactic peptide, suggesting that it has important immuno-modulatory functions. NGAL has been described as an inflammatory protein; it is released into the circulation as a result of the inflammatory activation of leukocytes initiated by the extra-corporeal circulation. In addition, NGAL synthesis is induced in epithelial cells in inflammatory and neoplastic colorectal diseases. In conclusion, NGAL may serve as a scavenger of bacterial products to function in the anti-inflammatory process.
CITATIONS
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Synergistic effects of lipopolysaccharide and rotenone on dopamine neuronal damage in rats
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WB
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Rat
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Citation
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