PRODUCT CODE: ET7108-51

Lipin 1 Recombinant Rabbit Monoclonal Antibody [JG37-69] (ET7108-51)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

Western blot analysis of Lipin 1 on different cell lysates using anti-Lipin 1 at 1/500 dilution.<br />
 Positive control:<br />
 Line 1: K562 <br />
        Line 2: SH-SY-5Y<br />
 Line 3: Mouse lung
  • Western blot analysis of Lipin 1 on different cell lysates using anti-Lipin 1 at 1/500 dilution.<br />
 Positive control:<br />
 Line 1: K562 <br />
        Line 2: SH-SY-5Y<br />
 Line 3: Mouse lung
  • ICC staining Lipin 1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Lipin 1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Lipin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Lipin 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using anti-Lipin 1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of PC-3M cells with Lipin 1 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Lipin 1 on different cell lysates using anti-Lipin 1 at 1/500 dilution.
Positive control:
Line 1: K562
Line 2: SH-SY-5Y
Line 3: Mouse lung

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Lipin 1 Recombinant Rabbit Monoclonal Antibody [JG37-69] (ET7108-51)

Immunogen

Recombinant protein within human lipin 1 aa 50-250.

Host

Rabbit

Positive Control

K562, SH-SY-5Y, HUVEC, PC-3M, human liver cancer tissue, human colon tissue, human skeletal muscle tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG37-69

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

98 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Lipin 1

SYNONYMS

EC=3.1.3.4 antibody; KIAA0188 antibody; Lipin-1 antibody; Lpin1 antibody; LPIN1_HUMAN antibody; PAP1 antibody; Phosphatidate phosphatase LPIN1 antibody

SEQUENCE SIMILARITIES

Belongs to the lipin family.

TISSUE SPECIFICITY

Specifically expressed in skeletal muscle. Also abundant in adipose tissue. Lower levels in some portions of the digestive tract.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated at multiple sites in response to insulin. Phosphorylation is controlled by the mTOR signaling pathway. Phosphorylation is decreased by epinephrine. Phosphorylation may not directly affect the catalytic activity but may regulate the localization. Dephosphorylated by the CTDNEP1-CNEP1R1 complex (By similarity).; Sumoylated.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm. Endoplasmic reticulum.

FUNCTION

Plays important roles in controlling the metabolism of fatty acids at different levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis in the reticulum endoplasmic membrane. Acts also as a nuclear transcriptional coactivator for PPARGC1A/PPARA to modulate lipid metabolism gene expression (By similarity). Is involved in adipocyte differentiation. May also be involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol (By similarity).