PRODUCT CODE: M1301-1

LIN28A Mouse Monoclonal Antibody [A4-A6] (M1301-1)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis on NCCIT cell lysates using anti-LIN28A Mouse mAb (Cat. #M1301-1).
  • Western blot analysis on NCCIT cell lysates using anti-LIN28A Mouse mAb (Cat. #M1301-1).
  • Immunofluorescent staining of F9 cells using anti-LIN28A Mouse mAb (Cat. #M1301-1).
  • Immunohistochemical analysis of paraffin- embedded human kidney cancer tissue using anti-LIN28A Mouse mAb (Cat. #M1301-1).
Western blot analysis on NCCIT cell lysates using anti-LIN28A Mouse mAb (Cat. #M1301-1).

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

LIN28A Mouse Monoclonal Antibody [A4-A6] (M1301-1)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

NCCIT F9

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A4-A6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

26 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:10,000

  • ICC

  • 1:100

  • IHC-P

  • 1:100

TARGET

UNIPROT #

PROTEIN NAME

LIN28A

SYNONYMS

CSDD1 antibody; FLJ12457 antibody; LIN 28 antibody; Lin 28 homolog A (C. elegans) antibody; Lin-28A antibody; LIN28 antibody; LIN28A antibody; LN28A_HUMAN antibody; Protein lin-28 homolog A antibody; ZCCHC1 antibody; zinc finger CCHC domain containing 1 antibody; Zinc finger CCHC domain-containing protein 1 antibody

SEQUENCE SIMILARITIES

Belongs to the lin-28 family.

TISSUE SPECIFICITY

Expressed in embryonic stem cells, placenta and testis. Tends to be up-regulated in HER2-overexpressing breast tumors.

DEVELOPMENTAL STAGE

Expressed in fetal liver. Expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.

SUBCELLULAR LOCATION

Cytoplasm Nucleus

FUNCTION

LIN28A is conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs. LIN28A is localized to the periendoplasmic reticulum (ER) area and inhibits translation of mRNAs that are destined for the ER, reducing the synthesis of transmembrane proteins, ER or Golgi lumen proteins, and secretory proteins. Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells.