PRODUCT CODE: ER1902-83

LIM1 Rabbit Polyclonal Antibody (ER1902-83)

  • Zebrafish

Applications

  • WB

  • IHC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis of LIM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-83, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A431 cell lysate <br />
Lane 2: Zebrafish tissue lysate
  • Western blot analysis of LIM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-83, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: A431 cell lysate <br />
Lane 2: Zebrafish tissue lysate
  • Immunohistochemical analysis of paraffin-embedded rat brian tissue using anti-LIM1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-83, 1/200)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse brian tissue using anti-LIM1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-83, 1/200)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of LIM1 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-83, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; blcak).
Western blot analysis of LIM1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-83, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A431 cell lysate
Lane 2: Zebrafish tissue lysate

Applications

  • WB

  • IHC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

LIM1 Rabbit Polyclonal Antibody (ER1902-83)

Immunogen

Synthetic peptide within zebrafish lim1 aa 144-193 / 405.

Host

Rabbit

Positive Control

A431 cell lysate, zebrafish tissue lysate, rat brian tissue, mouse brian tissue, MCF-7.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

45 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

LIM1

SYNONYMS

hLim-1 antibody; Homeo box protein Lim 1 antibody; Homeo box protein Lim1 antibody; Homeobox protein Lim 1 antibody; Homeobox protein Lim-1 antibody; Homeobox protein Lim1 antibody; LHX 1 antibody; LHX1 antibody; LHX1_HUMAN antibody; LIM 1 antibody; LIM homeo box 1 antibody; LIM homeo box protein 1 antibody; LIM homeobox 1 antibody; LIM homeobox protein 1 antibody; LIM-1 antibody; LIM/homeobox protein Lhx 1 antibody; LIM/homeobox protein Lhx1 antibody; MGC126723 antibody; MGC138141 antibody

TISSUE SPECIFICITY

Expressed in the brain, thymus, and tonsils. Expressed in samples from patients with chronic myeloid leukemia (CML) and in 58% of acute myeloid leukemia (AML) cell lines.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The Lim gene family is a subfamily of homeobox genes. The homeobox genes are essential in organizing the body plan of an organism and all contain the same conserved homeodomain of amino acids.Evidence that Lim-1 is essential to a developing organism is its conservation throughout evolution and presence in a variety of organisms.The Lim-1 gene encodes a transcription factor which binds to the DNA of specific genes and functions to produce the needed gene product for development of the organism. Lim-1 is important during early molecular development and is required in both primitive streak-derived tissue and visceral endoderm of the early embryo for development of a head. Studies done using mutant organisms without the Lim gene results in organisms that develop no head structure at all support the essential role of the Lim-1 gene in formation of the head. This gene has also been shown to play a crucial role in the formation of the female reproductive tract.The gene is expressed in the developing Müllerian duct of females, and when the gene is knocked out no reproductive tract forms. Recent studies have shown that Lim-1 mutations may be one cause of the Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome. MRKH is characterized by defective development, or absence, of the uterus and upper part of the vagina in women with normal ovaries and karyotype.