LIM Kinase 1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH08-68] (HA751233)
Catalog# HA751233
Rabbit Monoclonal to LIM Kinase 1
Tested for
-
WB
-
IHC-P
-
FC
Reacts with
-
Human
-
Mouse
-
Rat
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of LIM Kinase 1 on different lysates with Rabbit anti-LIM Kinase 1 antibody (HA751233) at 1/4,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: COS-1 cell lysate
Lane 5: Mouse liver tissue lysate (negative)
Lane 6: Mouse brain tissue lysate
Lane 7: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 73 kDa
Observed band size: 70 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751233) at 1/4,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of LIM Kinase 1 on different lysates with Rabbit anti-LIM Kinase 1 antibody (HA751233) at 1/4,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: COS-1 cell lysate
Lane 5: Mouse liver tissue lysate (negative)
Lane 6: Mouse brain tissue lysate
Lane 7: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 73 kDa
Observed band size: 70 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751233) at 1/4,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.