Positive control:
Lane 1: mouse spinal cord tissue lysate
Lane 2: human skeletal muscle tissue lysate
Applications
-
WB
-
IP
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
LGR5/GPR49 Recombinant Rabbit Monoclonal Antibody [SU32-04] (ET1608-18)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Mouse spinal cord tissue lysate, human skeletal muscle tissue lysate, mouse skeletal muscle tissue, SH-SY5Y.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU32-04
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
100 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
LGR5/GPR49
SYNONYMS
FEX antibody; G protein coupled receptor 49 antibody; G protein coupled receptor 67 antibody; g protein-coupled receptor fex antibody; G-protein coupled receptor 49 antibody; G-protein coupled receptor 67 antibody; G-protein coupled receptor HG38 antibody; GPR 49 antibody; GPR 67 antibody; GPR49 antibody; GPR67 antibody; GRP 49 antibody; GRP49 antibody; HG 38 antibody; HG38 antibody; Leucine rich repeat containing G protein coupled receptor 5 antibody; Leucine-rich repeat-containing G-protein coupled receptor 5 antibody; LGR 5 antibody; LGR5 antibody; LGR5_HUMAN antibody; MGC117008 antibody; Orphan G protein coupled receptor HG38 antibody
SEQUENCE SIMILARITIES
Belongs to the G-protein coupled receptor 1 family.
TISSUE SPECIFICITY
Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.
SUBCELLULAR LOCATION
trans-Golgi network membrane, Cell membrane.
FUNCTION
G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors or heptahelical receptors, interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers, such as diacylglycerol, cyclic AMP, inositol phosphates and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. LGR5 (leucine-rich repeat-containing G-protein coupled receptor 5), also known as GPR49 or GPR67, is a 907 amino acid multi-pass membrane protein that contains 17 leucine-rich repeats and belongs to the G protein-coupled receptor family. Expressed in placenta, skeletal muscle and spinal cord, LGR5 functions as an orphan receptor that is thought to play an important role in embryonic growth control and cellular differentiation. Overexpression of LGR5 is associated with increased tumor susceptibility and malignant transformation, implicating LGR5 as a potent tumor-inducing protein.