PRODUCT CODE: ET1606-47

LDL Receptor Recombinant Rabbit Monoclonal Antibody [SJ0197] (ET1606-47)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of LDL Receptor on MCF-7 lysates using anti-LDL Receptor antibody at 1/1,000 dilution.
  • Western blot analysis of LDL Receptor on MCF-7 lysates using anti-LDL Receptor antibody at 1/1,000 dilution.
  • ICC staining LDL Receptor in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining LDL Receptor in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining LDL Receptor in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with LDL Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of LDL Receptor on MCF-7 lysates using anti-LDL Receptor antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

LDL Receptor Recombinant Rabbit Monoclonal Antibody [SJ0197] (ET1606-47)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A549, MCF-7, Hela, HepG2, human liver tissue, human liver cancer tissue, human lung tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SJ0197

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

140 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

LDL Receptor

SYNONYMS

FH antibody; FHC antibody; LDL R antibody; LDL receptor antibody; LDLCQ2 antibody; Ldlr antibody; LDLR_HUMAN antibody; Low Density Lipoprotein Receptor antibody; Low density lipoprotein receptor class A domain containing protein 3 antibody; Low density lipoprotein receptor familial hypercholesterolemia antibody; Low-density lipoprotein receptor antibody

SEQUENCE SIMILARITIES

Belongs to the LDLR family.

POST-TRANSLATIONAL MODIFICATION

N- and O-glycosylated.; Ubiquitinated by MYLIP leading to degradation.

SUBCELLULAR LOCATION

Membrane, Cell surface, Endosome.

FUNCTION

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family, which includes LDLR, LRP, Megalin, VLDLR, and ApoER2. The LDL receptor family is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats, and an O-linked sugar domain. The LDL receptor is a cell surface transmembrane protein that mediates the uptake of low density lipoprotein and its degradation in the lysosome, which provides cholesterol to cells. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. Mutations in LDLR cause the autosomal dominant disease, familial hypercholesterolemia (FH), which promotes premature coronary atherosclerosis.