LDHA Rabbit Polyclonal Antibody (ER00702)
Catalog# ER00702
Rabbit Polyclonal to LDHA
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WB
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IHC-P
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FC
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: A431 cell lysate
Lane 2: MCF7 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: C6 cell lysate
Lane 6: PC-12 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 35 kDa
Exposure time: 20 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER00702) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
LDHA Rabbit Polyclonal Antibody (ER00702)
Immunogen
Synthetic peptide within human ldha aa 1-50 / 332.
Host
Rabbit
Positive Control
A431 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, PC-12 cell lysate, human liver tissue, mouse liver tissue, rat liver tissue, A431, RAW264.7, C6.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IHC-P
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1:1,000
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FC
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1:1,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 37 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
LDHA
SYNONYMS
Cell proliferation-inducing gene 19 protein antibody;GSD11 antibody;L lactate dehydrogenase A chain antibody;L-lactate dehydrogenase A chain antibody;l7R2 antibody;Lactate dehydrogenase 1, A chain antibody;Lactate dehydrogenase A antibody;Lactate dehydrogenase A4 antibody;Lactate dehydrogenase M antibody;LDH A antibody;LDH M antibody;LDH muscle subunit antibody;LDH muscle subunit; M LDH antibody;LDH-A antibody;LDH-M antibody;LDH1 antibody;ldha antibody;LDHA_HUMAN antibody;LDHM antibody;OTTMUSP00000017774 antibody;PIG19 antibody;Proliferation-inducing gene 19 antibody;Renal carcinoma antigen NY-REN-59 antibody
SEQUENCE SIMILARITIES
Belongs to the LDH/MDH superfamily. LDH family.
POST-TRANSLATIONAL MODIFICATION
ISGylated.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Lactate dehydrogenase (LDH) is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. In medicine, LDH is often used as a marker of tissue breakdown as LDH is abundant in red blood cells and can function as a marker for hemolysis. In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor.
CITATIONS
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Dong, J., Guo, C., Yang, Z., Wu, Y., & Zhang, C.
Follicle-Stimulating Hormone Alleviates Ovarian Aging by Modulating Mitophagy- and Glycophagy-Based Energy Metabolism in Hens
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WB
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IF
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Hen
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Citation
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