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PRODUCT CODE: ER00702

LDHA Rabbit Polyclonal Antibody (ER00702)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of LDHA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER00702, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: Human tonsil tissue lysate<br /> Lane 2: NIH/3T3 cell lysate<br /> Positive control: <br /> Lane 1: A549 cell lysate<br /> Lane 2: Hela cell lysate<br /> Lane 3: A431 cell lysate<br /> Lane 4: 293 cell lysate<br /> Lane 5: Jurkat cell lysate<br /> Lane 6: MCF-7 cell lysate<br /> Lane 7: Human liver tissue lysate<br /> Lane 8: Human kidney tissue lysate<br /> Lane 9: Human brain tissue lysate<br /> Lane 10: Human thymus tissue lysate
  • Western blot analysis of LDHA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER00702, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: Human tonsil tissue lysate<br /> Lane 2: NIH/3T3 cell lysate<br /> Positive control: <br /> Lane 1: A549 cell lysate<br /> Lane 2: Hela cell lysate<br /> Lane 3: A431 cell lysate<br /> Lane 4: 293 cell lysate<br /> Lane 5: Jurkat cell lysate<br /> Lane 6: MCF-7 cell lysate<br /> Lane 7: Human liver tissue lysate<br /> Lane 8: Human kidney tissue lysate<br /> Lane 9: Human brain tissue lysate<br /> Lane 10: Human thymus tissue lysate
  • All lanes: Western blot analysis of LDHA with anti-LDHA antibody (ER00702) at 1:500 dilution.<br /> Lane 1: Wild-type Hela whole cell lysate (10 µg).<br /> Lane 2/3: LDHA knockdown Hela whole cell lysate (10 µg).<br /> <br /> ER00702 was shown to specifically react with LDHA in wild-type Hela cells. Weakened bands were observed when LDHA knockdown samples were tested. Wild-type and LDHA knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER00702, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-LDHA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER00702, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-LDHA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER00702, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-LDHA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER00702, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-LDHA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER00702, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-LDHA antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER00702, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of LDHA on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER00702, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human tonsil tissue lysate
Lane 2: NIH/3T3 cell lysate
Positive control:
Lane 1: A549 cell lysate
Lane 2: Hela cell lysate
Lane 3: A431 cell lysate
Lane 4: 293 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF-7 cell lysate
Lane 7: Human liver tissue lysate
Lane 8: Human kidney tissue lysate
Lane 9: Human brain tissue lysate
Lane 10: Human thymus tissue lysate

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

LDHA Rabbit Polyclonal Antibody (ER00702)

Immunogen

Synthetic peptide within human ldha aa1-50 / 332.

Host

Rabbit

Positive Control

Human tonsil tissue lysate, NIH/3T3 cell lysate, A549 cell lysate, Hela cell lysate, A431 cell lysate, 293 cell lysate, Jurkat cell lysate, MCF-7 cell lysate, human liver tissue lysate, human kidney tissue lysate, human brain tissue lysate, human thymus tissue lysate, rat liver tissue, rat skeletal muscle tissue, human liver tissue, human breast carcinoma tissue, mouse liver tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified.

MOLECULAR WEIGHT

37 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

P00338

PROTEIN NAME

LDHA

SYNONYMS

Cell proliferation-inducing gene 19 protein antibody; GSD11 antibody; L lactate dehydrogenase A chain antibody; L-lactate dehydrogenase A chain antibody; l7R2 antibody; Lactate dehydrogenase 1, A chain antibody; Lactate dehydrogenase A antibody; Lactate dehydrogenase A4 antibody; Lactate dehydrogenase M antibody; LDH A antibody; LDH M antibody; LDH muscle subunit antibody; LDH muscle subunit; M LDH antibody; LDH-A antibody; LDH-M antibody; LDH1 antibody; ldha antibody; LDHA_HUMAN antibody; LDHM antibody; OTTMUSP00000017774 antibody; PIG19 antibody; Proliferation-inducing gene 19 antibody; Renal carcinoma antigen NY-REN-59 antibody

SEQUENCE SIMILARITIES

Belongs to the LDH/MDH superfamily. LDH family.

POST-TRANSLATIONAL MODIFICATION

ISGylated.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Lactate dehydrogenase (LDH) is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. In medicine, LDH is often used as a marker of tissue breakdown as LDH is abundant in red blood cells and can function as a marker for hemolysis. In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor.