PRODUCT CODE: ER00702

LDHA Rabbit Polyclonal Antibody (ER00702)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of LDHA on different cell lysates using anti-LDHA antibody at 1/500 dilution.<br />
Positive control:   <br />
Lane 1: A549             <br />
Lane 2: Hela <br />
Lane 3: A431             <br />
Lane 4: 293 <br />
Lane 5: Jurkat           <br />
Lane 6: MCF-7 <br />
Lane 7: Human liver      <br />
Lane 8: Human kidney <br />
Lane 9: Human brain       <br />
Lane 10: Human thymus
  • Western blot analysis of LDHA on different cell lysates using anti-LDHA antibody at 1/500 dilution.<br />
Positive control:   <br />
Lane 1: A549             <br />
Lane 2: Hela <br />
Lane 3: A431             <br />
Lane 4: 293 <br />
Lane 5: Jurkat           <br />
Lane 6: MCF-7 <br />
Lane 7: Human liver      <br />
Lane 8: Human kidney <br />
Lane 9: Human brain       <br />
Lane 10: Human thymus
  • Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-LDHA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-LDHA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-LDHA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-LDHA antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-LDHA antibody. Counter stained with hematoxylin.
Western blot analysis of LDHA on different cell lysates using anti-LDHA antibody at 1/500 dilution.
Positive control:
Lane 1: A549
Lane 2: Hela
Lane 3: A431
Lane 4: 293
Lane 5: Jurkat
Lane 6: MCF-7
Lane 7: Human liver
Lane 8: Human kidney
Lane 9: Human brain
Lane 10: Human thymus

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

LDHA Rabbit Polyclonal Antibody (ER00702)

Immunogen

Synthetic peptide within n-terminal human ldha.

Host

Rabbit

Positive Control

A549, MCF-7, Hela, Jurkat, A431, 293, human liver tissue, human brain tissue, human kidney tissue, human thymus tissue, human breast cancer tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue, rat liver tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

37 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

LDHA

SYNONYMS

Cell proliferation-inducing gene 19 protein antibody; GSD11 antibody; L lactate dehydrogenase A chain antibody; L-lactate dehydrogenase A chain antibody; l7R2 antibody; Lactate dehydrogenase 1, A chain antibody; Lactate dehydrogenase A antibody; Lactate dehydrogenase A4 antibody; Lactate dehydrogenase M antibody; LDH A antibody; LDH M antibody; LDH muscle subunit antibody; LDH muscle subunit; M LDH antibody; LDH-A antibody; LDH-M antibody; LDH1 antibody; ldha antibody; LDHA_HUMAN antibody; LDHM antibody; OTTMUSP00000017774 antibody; PIG19 antibody; Proliferation-inducing gene 19 antibody; Renal carcinoma antigen NY-REN-59 antibody

SEQUENCE SIMILARITIES

Belongs to the LDH/MDH superfamily. LDH family.

POST-TRANSLATIONAL MODIFICATION

ISGylated.

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

Lactate dehydrogenase (LDH) is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. In medicine, LDH is often used as a marker of tissue breakdown as LDH is abundant in red blood cells and can function as a marker for hemolysis. In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor.