Positive control:
Lane 1: Human tonsil tissue lysate
Lane 2: NIH/3T3 cell lysate
Positive control:
Lane 1: A549 cell lysate
Lane 2: Hela cell lysate
Lane 3: A431 cell lysate
Lane 4: 293 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF-7 cell lysate
Lane 7: Human liver tissue lysate
Lane 8: Human kidney tissue lysate
Lane 9: Human brain tissue lysate
Lane 10: Human thymus tissue lysate
Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
LDHA Rabbit Polyclonal Antibody (ER00702)
Immunogen
Synthetic peptide within human ldha aa1-50 / 332.
Host
Rabbit
Positive Control
Human tonsil tissue lysate, NIH/3T3 cell lysate, A549 cell lysate, Hela cell lysate, A431 cell lysate, 293 cell lysate, Jurkat cell lysate, MCF-7 cell lysate, human liver tissue lysate, human kidney tissue lysate, human brain tissue lysate, human thymus tissue lysate, rat liver tissue, rat skeletal muscle tissue, human liver tissue, human breast carcinoma tissue, mouse liver tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Immunogen affinity purified.
MOLECULAR WEIGHT
37 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500
-
IHC-P
-
1:200
-
FC
-
1:100-1:200
TARGET
UNIPROT #
PROTEIN NAME
LDHA
SYNONYMS
Cell proliferation-inducing gene 19 protein antibody; GSD11 antibody; L lactate dehydrogenase A chain antibody; L-lactate dehydrogenase A chain antibody; l7R2 antibody; Lactate dehydrogenase 1, A chain antibody; Lactate dehydrogenase A antibody; Lactate dehydrogenase A4 antibody; Lactate dehydrogenase M antibody; LDH A antibody; LDH M antibody; LDH muscle subunit antibody; LDH muscle subunit; M LDH antibody; LDH-A antibody; LDH-M antibody; LDH1 antibody; ldha antibody; LDHA_HUMAN antibody; LDHM antibody; OTTMUSP00000017774 antibody; PIG19 antibody; Proliferation-inducing gene 19 antibody; Renal carcinoma antigen NY-REN-59 antibody
SEQUENCE SIMILARITIES
Belongs to the LDH/MDH superfamily. LDH family.
POST-TRANSLATIONAL MODIFICATION
ISGylated.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Lactate dehydrogenase (LDH) is an enzyme present in a wide variety of organisms, including plants and animals. It catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. In medicine, LDH is often used as a marker of tissue breakdown as LDH is abundant in red blood cells and can function as a marker for hemolysis. In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor.