PRODUCT CODE: M1006-6

LASS2 Mouse Monoclonal Antibody [B3-2] (M1006-6)

  • Zebrafish

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Zebrafish

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Western blot analysis on K562-AO2 cell lysates using anti-lass2 monoclonal antibody.
  • Western blot analysis on K562-AO2 cell lysates using anti-lass2 monoclonal antibody.
  • Western blot analysis of LASS2 in on hybrid fish (crucian-carp) brain tissue lysate using anti-LASS2 antibody at 1/500 dilution.
  • ICC staining LASS2 (red) in K562 cells.  Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-LASS2 antibody. Counter stained with hematoxylin.
Western blot analysis on K562-AO2 cell lysates using anti-lass2 monoclonal antibody.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Zebrafish

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

LASS2 Mouse Monoclonal Antibody [B3-2] (M1006-6)

Immunogen

Peptide

Host

Mouse

Positive Control

K562-AO2

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

B3-2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

45 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:500~1:1,000

TARGET

UNIPROT #

PROTEIN NAME

LASS2

SYNONYMS

Ceramide synthase 2 antibody; CerS 2 antibody; CerS2 antibody; CERS2_HUMAN antibody; FLJ10243 antibody; L3 antibody; LAG1 homolog ceramide synthase 2 antibody; LAG1 longevity assurance 2 antibody; LAG1 longevity assurance homolog 2 antibody; LASS 2 antibody; Longevity assurance (LAG1 S. cerevisiae) homolog 2 antibody; MGC987 antibody; SP 260 antibody; SP260 antibody; TMSG 1 antibody; TMSG1 antibody; Tumor metastasis suppressor antibody; Tumor metastasis suppressor gene 1 protein antibody; Tumor metastasis-suppressor gene 1 protein antibody

TISSUE SPECIFICITY

Expressed in kidney, liver, brain, heart, placenta and lung.

POST-TRANSLATIONAL MODIFICATION

Acetylated. Deacetylation by SIRT3 increases enzyme activity and promotes mitochondrial ceramide accumulation.; Phosphorylated at the C-terminus by CK2, leading to increase the ceramide synthase activity.

SUBCELLULAR LOCATION

Nuclear membrane

FUNCTION

The LAG1 gene encodes a protein that has sequence similarity to yeast longevity assurance gene 1. Mutation or overexpression of the related gene in yeast has been shown to alter yeast lifespan. Expression of LASS2 in hepatocellular carcinoma cell lines suppresses the growth of cancer cells.The human protein may play a role in the regulation of cell growth. Alternatively spliced transcript variants encoding the same protein have been described.