PRODUCT CODE: ET1701-94

LAMP1 Recombinant Rabbit Monoclonal Antibody [JJ0940] (ET1701-94)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of LAMP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-94, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: Jurkat cell lysate
  • Western blot analysis of LAMP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-94, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: Jurkat cell lysate
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-LAMP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-94, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of LAMP1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-94, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:40,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

LAMP1 Recombinant Rabbit Monoclonal Antibody [JJ0940] (ET1701-94)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Jurkat, Hela, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ0940

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

120 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

LAMP1

SYNONYMS

CD107 antigen like family member A antibody; CD107 antigen-like family member A antibody; CD107a antibody; CD107a antigen antibody; LAMP 1 antibody; LAMP-1 antibody; LAMP1 antibody; LAMP1_HUMAN antibody; LAMPA antibody; LGP120 antibody; lgpA antibody; Lysosomal membrane glycoprotein 120KD antibody; Lysosomal Associated Membrane Protein 1 antibody; Lysosome associated membrane glycoprotein 1 antibody; Lysosome-associated membrane glycoprotein 1 antibody; Lysosome-associated membrane protein 1 antibody; OTTHUMP00000040663 antibody

SEQUENCE SIMILARITIES

Belongs to the LAMP family.

POST-TRANSLATIONAL MODIFICATION

O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells.

SUBCELLULAR LOCATION

Cell membrane, Endosome membrane, Lysosome membrane, Late endosome.

FUNCTION

Lysosome-associated membrane proteins (LAMP) are glycosylated type I membrane proteins that play a role in the biogenesis of the pigment melanin. LAMP-1 (also designated CD107a) and LAMP-2 (also designated CD107b) are involved in a variety of functions, including cellular adhesion, and are thought to participate in the process of tumor invasion and metastasis. Newly synthesized LAMP-1 and LAMP-2 proteins are sorted at the trans-Golgi network and are transported intracellularly via a pathway that is distinct from the Clathrin-coated vesicles used for the mannose-6 phosphate receptor. LAMP-1 is expressed on the surface of Thrombin-activated but not resting platelets, and it is thought to be involved in the adhesive, prothrombic properties of these cells. Both LAMP-1 and LAMP-2 are involved in maintaining lysosome acidity and protecting the lysosomal membranes from autodigestion, and their expression is increased in patients with lysosomal storage disorders.

CITATIONS

  • Zhu, Xudong et al.

    Cellular Evidence and Source of Exosomes in the Biliary System of the Chinese Soft-Shelled Turtle, Pelodiscus sinensis. | Frontiers in Physiology [2019]