PRODUCT CODE: ET1701-99

Laminnin 5 alpha 3 Recombinant Rabbit Monoclonal Antibody [JJ0957] (ET1701-99)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

Western blot analysis of Laminnin 5 alpha 3 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Laminnin 5 alpha 3 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • ICC staining of Laminnin 5 alpha 3 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-99, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Laminnin 5 alpha 3 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1701-99, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of Laminnin 5 alpha 3 on A431 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-99, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • IF

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Laminnin 5 alpha 3 Recombinant Rabbit Monoclonal Antibody [JJ0957] (ET1701-99)

Immunogen

Recombinant protein within human laminnin 5 alpha 3 aa 50-150.

Host

Rabbit

Positive Control

A431, Hela, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ0957

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

367 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

Laminnin 5 alpha 3

SYNONYMS

E170 antibody; Epiligrin 170 kDa subunit antibody; Epiligrin subunit alpha antibody; Kalinin subunit alpha antibody; LAMA3 antibody; LAMA3A antibody; Laminin 5 alpha3 antibody; Laminin A3 antibody; laminin alpha 3 antibody; laminin alpha3 antibody; Laminin subunit alpha-3 antibody; Laminin-5 subunit alpha antibody; Laminin-6 subunit alpha antibody; Laminin-7 subunit alpha antibody; laminin5 alpha 3 antibody; Laminin5 alpha3 antibody; LAMNA antibody; LOCS antibody; Nicein subunit alpha antibody

TISSUE SPECIFICITY

Skin; respiratory, urinary, and digestive epithelia and in other specialized tissues with prominent secretory or protective functions. Epithelial basement membrane, and epithelial cell tongue that migrates into a wound bed. A differential and focal expression of the subunit alpha-3 is observed in the CNS.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Laminins are heterotrimeric, noncollagenous glycoproteins composed of alpha, beta, and gamma chains. Through interactions with integrins, dystroglycan and other receptors, laminins contribute to cell differentiation, cell shape and migration, and maintenance of tissue phenotypes and survival. Laminin alpha 3/Laminin-5, also known as epiligrin, includes alpha 3, beta 3, and gamma 2 subunits. It is abundant in transitional epithelium, stratified squamous epithelia, lung mucosa and other epithelial glands and contributes to initiation and maintenance of epithelial cell anchorage to the underlying connective tissue. Within aa 21?1713 of the alpha 3 subunit, human and mouse share 77% amino acid sequence identity.