PRODUCT CODE: ET1703-51

L1CAM Recombinant Rabbit Monoclonal Antibody [JM11-05] (ET1703-51)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of L1CAM on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: Human brain tissue lysate
  • Western blot analysis of L1CAM on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: Human brain tissue lysate
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-L1CAM antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-L1CAM antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-51, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of L1CAM on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-51, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: Human brain tissue lysate

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

L1CAM Recombinant Rabbit Monoclonal Antibody [JM11-05] (ET1703-51)

Immunogen

Recombinant protein within human l1cam aa 900-1120.

Host

Rabbit

Positive Control

Hela, human brain, human kidney tissue, human thyroid tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-05

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

85/250 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

L1CAM

SYNONYMS

Antigen identified by monoclonal antibody; R1 antibody; CAML1 antibody; CD171 antibody; CD171 antigen antibody; HSAS antibody; HSAS1 antibody; Hyd antibody; L1 antibody; L1 cell adhesion molecule antibody; L1-NCAM antibody; L1cam antibody; L1CAM_HUMAN antibody; MASA antibody; MIC5 antibody; N CAML1 antibody; N-CAM-L1 antibody; NCAM-L1 antibody; NCAML1 antibody; Nerve-growth factor-inducible large external glycoprotein antibody; Neural cell adhesion molecule L1 antibody; NILE antibody; OTTHUMP00000025992 antibody; S10 antibody; SPG1 antibody

SEQUENCE SIMILARITIES

Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

Cell adhesion molecules are a family of closely related cell surface glycoproteins involved in cell-cell interactions during growth and are thought to play an important role in embryogenesis and development. Neuronal cell adhesion molecule (NCAM) expression is observed in a variety of human tumors, including neuroblastomas, rhabdomyosarcomas, Wilm's tumors, Ewing's sarcomas and some primitive myeloid malignancies. The NCAM-L1 adhesion molecule (CD171) plays an important role in axon guidance and cell migration in the nervous system. The presence of NCAM-L1 might contribute to tumor progression by promoting cell adhesion and migration and is known to be expressed by neurons, neuroblastomas and other malignant tumors.