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PRODUCT CODE: ER1802-91

KV4.3 Rabbit Polyclonal Antibody (ER1802-91)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of Kv4.3 on different lysates using anti-Kv4.3 antibody at 1/1,000 dilution.<br /> Positive control:<br /> Lane 1: MCF-7 <br /> Lane 2: Mouse marrow
  • Western blot analysis of Kv4.3 on different lysates using anti-Kv4.3 antibody at 1/1,000 dilution.<br /> Positive control:<br /> Lane 1: MCF-7 <br /> Lane 2: Mouse marrow
  • ICC staining Kv4.3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Kv4.3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Kv4.3 in MG-63 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Kv4.3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of MG-63 cells with Kv4.3 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Kv4.3 on different lysates using anti-Kv4.3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: MCF-7
Lane 2: Mouse marrow

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

KV4.3 Rabbit Polyclonal Antibody (ER1802-91)

Immunogen

Synthetic peptide within human kv43 aa 435-484 / 655.

Host

Rabbit

Positive Control

MCF-7, mouse marrow tissue, LOVO, MG-63, human esophagus tissue, human placenta tissue, mouse cerebellum tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

73 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q9UK17

PROTEIN NAME

KV4.3

SYNONYMS

KCND 3 antibody; KCND 3L antibody; KCND 3S antibody; Kcnd3 antibody; KCND3_HUMAN antibody; KCND3L antibody; KCND3S antibody; KSHIVB antibody; Kv 4.3 antibody; MGC14203 antibody; MGC142037 antibody; OTTHUMP00000063948 antibody; Potassium ioinic channel Kv4.3 antibody; Potassium voltage gated channel Shal related subfamily member 3 antibody; Potassium voltage gated channel subfamily D member 3 antibody; Potassium voltage-gated channel subfamily D member 3 antibody; Sha1 related potassium channel Kv4.3 antibody; Voltage gated K+ channel antibody; Voltage gated potassium channel Kv4.3 antibody; Voltage gated potassium channel subunit Kv4.3 antibody; Voltage-gated potassium channel subunit Kv4.3 antibody

SEQUENCE SIMILARITIES

Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.3/KCND3 sub-subfamily.

TISSUE SPECIFICITY

Highly expressed in heart and brain, in particular in cortex, cerebellum, amygdala and caudate nucleus. Detected at lower levels in liver, skeletal muscle, kidney and pancreas. Isoform 1 predominates in most tissues. Isoform 1 and isoform 2 are detected at similar levels in brain, skeletal muscle and pancreas.

POST-TRANSLATIONAL MODIFICATION

Regulated through phosphorylation at Ser-569 by CaMK2D.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.