Ku80 Rabbit Polyclonal Antibody (R1511-17)

UNIPROT #

P13010

PROTEIN NAME

Ku80

SYNONYMS

86 kDa subunit of Ku antigen antibody;ATP dependent DNA helicase 2 subunit 2 antibody;ATP dependent DNA helicase II 80 kDa subunit antibody;ATP dependent DNA helicase II 86 Kd subunit antibody;ATP dependent DNA helicase II antibody;ATP-dependent DNA helicase 2 subunit 2 antibody;ATP-dependent DNA helicase II 80 kDa subunit antibody;CTC box binding factor 85 kDa antibody;CTC box-binding factor 85 kDa subunit antibody;CTC85 antibody;CTCBF antibody;DNA repair protein XRCC5 antibody;KARP 1 antibody;KARP1 antibody;Ku 80 antibody;Ku autoantigen 80kDa antibody;Ku80 antibody;Ku86 antibody;Ku86 autoantigen related protein 1 antibody;KUB 2 antibody;KUB2 antibody;Lupus Ku autoantigen protein p86 antibody;NFIV antibody;Nuclear factor IV antibody;Thyroid lupus autoantigen antibody;Thyroid-lupus autoantigen antibody;TLAA antibody;X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining) antibody;X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) antibody;X-ray repair cross-complementing protein 5 antibody;Xray repair complementing defective repair in Chinese hamster cells 5 antibody;XRCC 5 antibody;XRCC5 antibody;XRCC5_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the ku80 family.

DEVELOPMENTAL STAGE

Expression increases during promyelocyte differentiation.

POST-TRANSLATIONAL MODIFICATION

ADP-ribosylated by PARP3.; Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.; Sumoylated.; Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination following DNA damage, leading to its degradation and removal from DNA damage sites. Ubiquitinated by RNF138, leading to remove the Ku complex from DNA breaks.

SUBCELLULAR LOCATION

Nucleus. Chromosome.

FUNCTION

Single stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription.