Positive control:
Lane 1: HepG2 cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: Rat brain tissue lysate
Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
KPTN Rabbit Polyclonal Antibody (ER2001-17)
Immunogen
Synthetic peptide within n-terminal human kptn.
Host
Rabbit
Positive Control
HepG2 cell lysate, SH-SY5Y cell lysate, rat brain tissue lysate, human placenta tissue, mouse testis tissue, SH-SY5Y.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified.
MOLECULAR WEIGHT
48 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
KPTN
SYNONYMS
2E4 antibody; actin associated protein 2E4 antibody; actin binding protein antibody; Actin-associated protein 2E4 antibody; Kaptin antibody; Kptn antibody; KPTN protein antibody; KPTN_HUMAN antibody
SUBCELLULAR LOCATION
Lysosome membrane, lamellipodium, stereocilium.
FUNCTION
As part of the KICSTOR complex functions in the amino acid-sensing branch of the TORC1 signaling pathway. Recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes and allows its interaction with GATOR2 and the RAG GTPases. Functions upstream of the RAG GTPases and is required to negatively regulate mTORC1 signaling in absence of amino acids. In absence of the KICSTOR complex mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also probably involved in the regulation of mTORC1 by glucose. Mutations in this gene result in an autosomal recessive form of intellectual disability. Alternatively spliced transcript variants have been found for this gene.