KMT6 / EZH2 Recombinant Rabbit Monoclonal Antibody [JJ089-9] (ET1701-56)

UNIPROT #

Q15910

PROTEIN NAME

KMT6/EZH2

SYNONYMS

Enhancer of zeste 2 antibody;enhancer of zeste 2 polycomb repressive complex 2 subunit antibody;Enhancer of zeste homolog 2 (Drosophila) antibody;Enhancer of zeste homolog 2 antibody;Enhancer of zeste, Drosophila, homolog 2 antibody;ENX 1 antibody;Enx 1h antibody;ENX-1 antibody;ENX1 antibody;Enx1h antibody;EZH 2 antibody;EZH1 antibody;EZH2 antibody;EZH2_HUMAN antibody;EZH2b antibody;Histone-lysine N-methyltransferase EZH2 antibody;KMT 6 antibody;KMT6 antibody;KMT6A antibody;Lysine N-methyltransferase 6 antibody;MGC9169 antibody;WVS antibody;WVS2 antibody

SEQUENCE SIMILARITIES

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. EZ subfamily.

TISSUE SPECIFICITY

Expressed in many tissues. Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis.

DEVELOPMENTAL STAGE

Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary.

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by AKT1. Phosphorylation by AKT1 reduces methyltransferase activity. Phosphorylation at Thr-345 by CDK1 and CDK2 promotes maintenance of H3K27me3 levels at EZH2-target loci, thus leading to epigenetic gene silencing.; Sumoylated.; Glycosylated: O-GlcNAcylation at Ser-75 by OGT increases stability of EZH2 and facilitates the formation of H3K27me3 by the PRC2/EED-EZH2 complex.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Displays a preference for substrates with less methylation, loses activity when progressively more methyl groups are incorporated into H3K27, H3K27me0 > H3K27me1 > H3K27me2. Compared to EZH1-containing complexes, it is more abundant in embryonic stem cells and plays a major role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1, CDKN2A and retinoic acid target genes. EZH2 can also methylate non-histone proteins such as the transcription factor GATA4 and the nuclear receptor RORA. Regulates the circadian clock via histone methylation at the promoter of the circadian genes. Essential for the CRY1/2-mediated repression of the transcriptional activation of PER1/2 by the CLOCK-ARNTL/BMAL1 heterodimer; involved in the di and trimethylation of 'Lys-27' of histone H3 on PER1/2 promoters which is necessary for the CRY1/2 proteins to inhibit transcription.