PRODUCT CODE: ER1803-98

Kir6.2 Rabbit Polyclonal Antibody (ER1803-98)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Kir6.2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Human liver tissue lysate<br />
Lane 2: Mouse liver tissue lysate
  • Western blot analysis of Kir6.2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Human liver tissue lysate<br />
Lane 2: Mouse liver tissue lysate
  • ICC staining of Kir6.2 in LoVo cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (ER1803-98) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Kir6.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-98) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Kir6.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-98) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Kir6.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-98) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Western blot analysis of Kir6.2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human liver tissue lysate
Lane 2: Mouse liver tissue lysate

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Kir6.2 Rabbit Polyclonal Antibody (ER1803-98)

Immunogen

Recombinant protein within human kir62 aa 184-323 / 390.

Host

Rabbit

Positive Control

Human liver tissue, mouse liver tissue, Lovo, mouse pancreas tissue, human prostate cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

44 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:1,000

  • ICC:1:50-1:200

  • IHC-P:1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Kir6.2

SYNONYMS

ATP sensitive inward rectifier potassium channel 11 antibody; Beta cell inward rectifier subunit antibody; BIR antibody; HHF 2 antibody; HHF2 antibody; IKATP antibody; Inward rectifier K(+) channel Kir6.2 antibody; Inwardly rectifying potassium channel KIR6.2 antibody; IRK 11 antibody; IRK11 antibody; KCNJ11 antibody; Kir 6.2 antibody; Kir6.2 antibody; MGC133230 antibody; PHHI antibody; Potassium channel inwardly rectifing subfamily J member 11 antibody; Potassium channel, inwardly rectifying subfamily J member 11 antibody; Potassium inwardly rectifying channel J11 antibody; TNDM 3 antibody; TNDM3 antibody

SEQUENCE SIMILARITIES

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ11 subfamily.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation by MAPK1 results in changes in channel gating that destabilize the closed states and reduce the ATP sensitivity.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow Potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and is found associated with the sulfonylurea receptor SUR. Mutations in this gene are a cause of familial persistent hyperinsulinemic hypoglycemia of infancy (PHHI), an autosomal recessive Disfect characterized by unregulated insulin secretion. Defects in this gene may also contribute to autosomal dominant non-insulin-dependent diabetes mellitus type II (NIDDM), transient neonatal diabetes mellitus type 3 (TNDM3), and permanent neonatal diabetes mellitus (PNDM). Multiple Alternative spliced ​​transcript variants that encode different protein isoforms have been described for this gene.