PRODUCT CODE: M0910-4

kinase D-interacting substrate 220 Mouse Monoclonal Antibody [3-B6] (M0910-4)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Mouse

  • Human

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Western blot analysis of kinase D-interacting substrate 220 on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0910-4, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of kinase D-interacting substrate 220 on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0910-4, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis on recombinant protein using anti-Kidins220 Mouse mAb (Cat. # M0910-4).
  • Immunofluorescent staining of F9 cells using anti-Kidins220 Mouse mAb (Cat. # M0910-4).
  • Immunohistochemical analysis of paraffin- embedded mouse brain tissue using anti-Kidins220 Mouse mAb (Cat. # M0910-4).
Western blot analysis of kinase D-interacting substrate 220 on K562 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0910-4, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Mouse

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

kinase D-interacting substrate 220 Mouse Monoclonal Antibody [3-B6] (M0910-4)

Immunogen

Protein

Host

Mouse

Positive Control

K562 cell lysates.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

3-B6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

199kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:1,000-1:10,000

  • ICC

  • 1:100

  • IHC-P

  • 1:50

TARGET

UNIPROT #

PROTEIN NAME

kinase D-interacting substrate 220

SUBCELLULAR LOCATION

Cell membrane

FUNCTION

Kinase D-interacting substrate 220 (Kidins220), also known as ARMS (ankyrin-rich membrane spanning) is an integral plasma membrane protein predominantly expressed in brain and neurons that presents a polarized location in neural cells, concentrating at the tip of extending neurites and in plasma membrane micro-domains corresponding to lipid rafts. The primary amino acid sequence of Kidins220 reveals 11 ankyrin repeats, 4 trans-membrane domains, a proline-rich region, a sterile-α motif (or SAM) domain, and a type I postsynaptic density-95/discs large/zonula occludens-1 (PDZ)-binding motif at the very C-terminus.