Applications
-
WB
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
KHSRP Mouse Monoclonal Antibody [A7-B0] (EM1701-97)
Immunogen
Synthetic peptide within human khsrp aa 625-674 / 711.
Host
Mouse
Positive Control
Jurkat, HT-29.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A7-B0
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
ProG purified.
MOLECULAR WEIGHT
73 kDa
Isotype
IgM
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
KHSRP
SYNONYMS
Far upstream element-binding protein 2 antibody; FBP2 antibody; FUBP2 antibody; FUBP2_HUMAN antibody; FUSE binding protein 2 antibody; FUSE-binding protein 2 antibody; KH type splicing regulatory protein (FUSE binding protein 2) antibody; KH type splicing regulatory protein antibody; KH type-splicing regulatory protein antibody; KHSRP antibody; KSRP antibody; p75 antibody
SEQUENCE SIMILARITIES
Belongs to the KHSRP family.
TISSUE SPECIFICITY
Detected in neural and non-neural cell lines.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-193 leads to the unfolding of the unstable KH domain 1, creating a site for 14-3-3 YWHAZ binding, which promotes nuclear localization and impairs the RNA degradation function.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Binds to the dendritic targeting element and may play a role in mRNA trafficking. Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.