KDM4A Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH03-67] (HA750901)
Catalog# HA750901
Rabbit Monoclonal to KDM4A
Tested for
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WB
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IF-Cell
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IHC-P
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FC
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of KDM4A on different lysates with Rabbit anti-KDM4A antibody (HA750901) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: A549 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HeLa cell lysate
Lane 4: HepG2 cell lysate
Lane 5: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 121 kDa
Observed band size: 150 kDa
Exposure time: Lane 1-5 (left): 2 minutes 16 seconds; ECL: K1801;
Exposure time: Lane 1-5 (right): 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750901) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HeLa cell lysate
Lane 4: HepG2 cell lysate
Lane 5: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 121 kDa
Observed band size: 150 kDa
Exposure time: Lane 1-5 (left): 2 minutes 16 seconds; ECL: K1801;
Exposure time: Lane 1-5 (right): 3 minutes; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750901) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.