Lane 1: Rat testis tissue lysate
Lane 2: Human placenta tissue lysate
Lane 3: Mouse testis tissue lysate
Lane 4: 293 cell lysate
Recombinant Rabbit monoclonal primary
KDEL Recombinant Rabbit Monoclonal Antibody [JB42-04] (ET7107-86)
Rat testis tissue, Mouse testis tissue, 293, HepG2, A549, 293T, rat epididymis tissue, human placenta tissue, human stomach cancer tissue, mouse small intestine tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A purified.
24/17 kDa(Predicted band size)
ER lumen protein retaining receptor 1 antibody; ERD2.1 antibody; ERD21_HUMAN antibody; KDEL endoplasmic reticulum protein retention receptor 1 antibody; KDEL receptor 1 antibody; Kdelr1 antibody; Putative MAPK-activating protein PM23 antibody
Belongs to the ERD2 family.
Phosphorylation by PKA at Ser-209 is required for endoplasmic reticulum retention function.
Endoplasmic reticulum. Membrane.
Soluble proteins in the endoplasmic reticulum (ER) contain a specific carboxy terminal sequence KDEL (Lys-Asp-Glu-Leu), and include the coat proteins required for vesicle budding from the ER, proteins that form retrograde vesicles on post-ER compartments, and integral membrane proteins that target vesicles to their correct destination. The retention of these soluble proteins in the ER depends on the interaction of the KDEL sequence with the corresponding KDEL receptor, also designated ERD2, in the Golgi apparatus. When KDEL proteins reach the Golgi complex, they are recognized by the KDEL receptor and transported retrograde in COPI-coated vesicles back to the ER. The small GTPase ADP-ribosylation factor 1 (ARF1), a regulator of vesicle transport, interacts with the KDEL receptor. Subsequently, this interaction allows the KDEL receptor to recruit a GTPase-activating protein (GAP) from the cytosol to membranes, which inactivates ARF1.