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PRODUCT CODE: ER1803-23

KCNA5 Rabbit Polyclonal Antibody (ER1803-23)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of KCNA5 on PANC-1 cell lysate using anti-KCNA5 antibody at 1/5,000 dilution.
  • Western blot analysis of KCNA5 on PANC-1 cell lysate using anti-KCNA5 antibody at 1/5,000 dilution.
  • ICC staining KCNA5 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining KCNA5 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining KCNA5 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-KCNA5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-KCNA5 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of PANC-1 cells with KCNA5 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of KCNA5 on PANC-1 cell lysate using anti-KCNA5 antibody at 1/5,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

KCNA5 Rabbit Polyclonal Antibody (ER1803-23)

Immunogen

Synthetic peptide within rat kcna5 aa 249-298 / 602.

Host

Rabbit

Positive Control

PANC-1, LOVO, SKOV-3, rat skeletal muscle tissue, rat heart tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

67 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

P22460

PROTEIN NAME

KCNA5

SYNONYMS

ATFB7 antibody; cardiac potassium channel antibody; HCK1 antibody; HK2 antibody; HPCN1 antibody; insulinoma and islet potassium channel antibody; KCNA5 antibody; KCNA5_HUMAN antibody; KV1.5 antibody; PCN1 antibody; potassium channel 1 antibody; Potassium channel, insulinoma and islet cell antibody; Potassium channel, voltage-gated, shaker-related subfamily, member 5 antibody; Potassium voltage gated channel subfamily A member 5 antibody; Potassium voltage-gated channel subfamily A member 5 antibody; Voltage-gated potassium channel HK2 antibody; voltage-gated potassium channel protein Kv1.5 antibody; Voltage-gated potassium channel subunit Kv1.5 antibody

SEQUENCE SIMILARITIES

Belongs to the potassium channel family. A (Shaker) (TC 1.A.1.2) subfamily. Kv1.5/KCNA5 sub-subfamily.

TISSUE SPECIFICITY

Pancreatic islets and insulinoma.

POST-TRANSLATIONAL MODIFICATION

Sumoylated on Lys-221, and Lys-536, preferentially with SUMO3. Sumoylation regulates the voltage sensitivity of the channel.

SUBCELLULAR LOCATION

Plasma membrane.

FUNCTION

Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Can form functional homotetrameric channels and heterotetrameric channels that contain variable proportions of KCNA1, KCNA2, KCNA4, KCNA5, and possibly other family members as well; channel properties depend on the type of alpha subunits that are part of the channel. Channel properties are modulated by cytoplasmic beta subunits that regulate the subcellular location of the alpha subunits and promote rapid inactivation. Homotetrameric channels display rapid activation and slow inactivation. May play a role in regulating the secretion of insulin in normal pancreatic islets. Isoform 2 exhibits a voltage-dependent recovery from inactivation and an excessive cumulative inactivation.