Junctional Adhesion Molecule 1 Recombinant Rabbit Monoclonal Antibody [SC60-07] (ET1610-90)
Catalog# ET1610-90
Rabbit Monoclonal to Junctional Adhesion Molecule 1
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WB
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IHC-P
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Human
Lane 1: HT-29 cell lysate (20 µg/Lane)
Lane 2: HUVEC cell lysate (20 µg/Lane)
Lane 3: TF-1 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: Human lung tissue lysate (40 µg/Lane)
Predicted band size: 33 kDa
Observed band size: 37 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-90) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Junctional Adhesion Molecule 1 Recombinant Rabbit Monoclonal Antibody [SC60-07] (ET1610-90)
Immunogen
Recombinant protein within human f11r aa 1-100.
Host
Rabbit
Positive Control
HT-29 cell lysate, HUVEC cell lysate, TF-1 cell lysate, HeLa cell lysate, Human lung tissue lysate, human breast cancer tissue, human endometrium tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC60-07
RRID
APPLICATION DILUTION
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WB
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1:2,000-1:5,000
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 33 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Junctional Adhesion Molecule 1
SYNONYMS
CD 321 antibody;CD321 antibody;CD321 antigen antibody;ESTM33 antibody;F11 receptor antibody;F11R antibody;JAM 1 antibody;JAM A antibody;JAM antibody;JAM-1 antibody;JAM-A antibody;JAM1 antibody;JAM1_HUMAN antibody;JAMA antibody;JCAM antibody;Jcam1 antibody;Junction adhesion molecule 1 antibody;Junction adhesion molecule, mouse, homolog of antibody;Junctional adhesion molecule 1 antibody;Junctional adhesion molecule A antibody;KAT antibody;Ly106 antibody;PAM 1 antibody;PAM-1 antibody;PAM1 antibody;Platelet adhesion molecule 1 antibody;Platelet adhesion molecule antibody;Platelet F11 receptor antibody;PRO301 antibody;UNQ264 antibody
SEQUENCE SIMILARITIES
Belongs to the immunoglobulin superfamily.
TISSUE SPECIFICITY
Expressed in endothelium, epithelium and leukocytes (at protein level).
POST-TRANSLATIONAL MODIFICATION
N-glycosylated.
SUBCELLULAR LOCATION
Cell junction, Cell membrane.
FUNCTION
Junctional adhesion molecule A is a protein that in humans is encoded by the F11R gene. It has also been designated as CD321 (cluster of differentiation 321). Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor.
CITATIONS
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Hong Changming,et al
Maternal resveratrol improves the intestinal health and weight gain of suckling piglets under high summer temperatures: The involvement of exosome-derived microRNAs and immunoglobin in colostrum
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WB
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Pig
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Citation
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Wang Xinyuan,et al
Age-, sex- and proximal-distal-resolved multi-omics identifies regulators of intestinal aging in non-human primates
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WB
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Mouse
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Citation
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