PRODUCT CODE: ET1612-68

JNK3 Recombinant Rabbit Monoclonal Antibody [SD082-09] (ET1612-68)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of JNK3 on 293T cells lysates using anti-JNK3 antibody at 1/1,000 dilution.
  • Western blot analysis of JNK3 on 293T cells lysates using anti-JNK3 antibody at 1/1,000 dilution.
  • ICC staining JNK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining JNK3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining JNK3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of Jurkat cells with JNK3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of JNK3 on 293T cells lysates using anti-JNK3 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

JNK3 Recombinant Rabbit Monoclonal Antibody [SD082-09] (ET1612-68)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T, NIH/3T3, Hela, SH-SY-5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD082-09

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

53 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • FC

  • 1:50-1:100

  • ICC/IF

  • 1:100-1:500

TARGET

UNIPROT #

PROTEIN NAME

JNK3

SYNONYMS

c Jun kinase 3 antibody; c-Jun N-terminal kinase 3 antibody; cJun N terminal kinase 3 antibody; FLJ12099 antibody; FLJ33785 antibody; JNK3 alpha protein kinase antibody; JNK3 antibody; JNK3A antibody; MAP kinase 10 antibody; MAP kinase antibody; MAP kinase p49 3F12 antibody; MAPK 10 antibody; Mapk10 antibody; MGC50974 antibody; mitogen activated protein kinase 10 antibody; Mitogen-activated protein kinase 10 antibody; MK10_HUMAN antibody; p493F12 antibody; p54bSAPK antibody; PRKM10 antibody; protein kinase mitogen activated 10 antibody; SAPK1b antibody; Stress activated protein kinase 1b antibody; stress activated protein kinase beta antibody; Stress activated protein kinase JNK3 antibody; Stress-activated protein kinase JNK3 antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

TISSUE SPECIFICITY

Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.

POST-TRANSLATIONAL MODIFICATION

Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme. MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially. Weakly autophosphorylated on threonine and tyrosine residues in vitro.; Palmitoylation regulates subcellular location and axonal development.

SUBCELLULAR LOCATION

Cytoplasm, Membrane, Nucleus, Mitochondrion.

FUNCTION

c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1a1,JNK1b1, JNK2a1, JNK2b1, and JNK3a1, which represent the p46 isoforms, and JNK1a2, JNK1b2, JNK2a2, JNK2b2, and JNK3b2, which represent the p54 isoforms.JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. JNK3 (MK10, MAPK10, PRKM10) is activated by pro-inflammatory cytokines and environmental stresss by phosphorylating transcription factors such as c-Jun and ATF2. This is important for AP-1 transcriptional activity regulation. JNK3 is crucial for neuronal apoptosis (stress-induced).