JNK2 Recombinant Rabbit Monoclonal Antibody [SC05-85] (ET1610-11)
Catalog# ET1610-11
Rabbit Monoclonal to JNK2
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WB
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IF-Cell
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
Western blot analysis of JNK2 on different lysates with Rabbit anti-JNK2 antibody (ET1610-11) at 1/2,000 dilution.
Lane 1: A549-si NT cell lysate
Lane 2: A549-si JNK2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 48/44 kDa
Observed band size: 48/44 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-11) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
JNK2 Recombinant Rabbit Monoclonal Antibody [SC05-85] (ET1610-11)
Immunogen
Synthetic peptide within human jnk2 aa 341-394 / 424.
Host
Rabbit
Positive Control
A549 cell lysate, 293T cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, C6 cell lysate, SHG-44 cell lysates, PC-12, NIH/3T3, human brain tissue, mouse brain tissue, rat brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC05-85
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Cell
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1:100
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IHC-P
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1:1,000
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FC
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1:1,000
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 48/44 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
JNK2
SYNONYMS
c Jun kinase 2 antibody;C Jun N terminal kinase 2 antibody;c-Jun N-terminal kinase 2 antibody;JNK 55 antibody;JNK-55 antibody;JNK2 alpha antibody;JNK2 antibody;JNK2 beta antibody;JNK2A antibody;JNK2alpha antibody;JNK2B antibody;JNK2BETA antibody;Jun kinase antibody;MAP kinase 9 antibody;MAPK 9 antibody;Mapk9 antibody;Mitogen activated protein kinase 9 antibody;Mitogen-activated protein kinase 9 antibody;MK09_HUMAN antibody;P54a antibody;p54aSAPK antibody;PRKM9 antibody;Protein kinase, mitogen-activated, 9 antibody;SAPK alpha antibody;SAPK antibody;SAPK1a antibody;Stress activated protein kinase 1a antibody;Stress-activated protein kinase JNK2 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
POST-TRANSLATIONAL MODIFICATION
Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Autophosphorylated in vitro.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
c-Jun N-terminal kinases (JNKs) phosphorylate and augment transcriptional activity of c-Jun. JNKs originate from three genes that yield 10 isoforms through alternative mRNA splicing, including JNK1α1, JNK1β1, JNK2α1, JNK2β1, and JNK3α1, which represent the p46 isoforms, and JNK1α2, JNK1β2, JNK2α2, JNK2β2, and JNK3β2, which represent the p54 isoforms.JNKs coordinate cell responses to stress and influence regulation of cell growth and transformation. The human JNK1 (PRKM8, SAPK1, MAPK8) gene maps to chromosome 10q11.22 and shares 83% amino acid identity with JNK2. JNK1 is necessary for normal activation and differentiation of CD4 helper T (TH) cells into TH1 and TH2 effector cells. Capsaicin activates JNK1 and p38 in ras-transformed human breast epithelial cells. Nitrogen oxides (NOx) upregulate JNK1 in addition to c-Fos, c-Jun, and other signaling kinases, including MEKK1 and p38.
CITATIONS
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Luo, P., Yan, H., Chen, X., Zhang, Y., Zhao, Z., Cao, J., Zhu, Y., Du, J., Xu, Z., Zhang, X., Zeng, S., Yang, B., Ma, S., & He, Q.
s-HBEGF/SIRT1 circuit-dictated crosstalk between vascular endothelial cells and keratinocytes mediates sorafenib-induced hand-foot skin reaction that can be reversed by nicotinamide. Cell research, 30(9), 779–793.
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WB
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IF
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IHC
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Human
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Citation
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