JNK1+JNK2+JNK3 Recombinant Rabbit Monoclonal Antibody [SA43-06] (ET1601-28)
Catalog# ET1601-28
Rabbit Monoclonal to JNK1+JNK2+JNK3
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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Human
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Mouse
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Rat
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Cow
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Monkey
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Zebrafish
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: MCF7 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: PC-12 cell lysate
Lane 7: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 48/53 kDa
Observed band size: 48/53 kDa
Exposure time: 35 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1601-28) at 1/2,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
JNK1+JNK2+JNK3 Recombinant Rabbit Monoclonal Antibody [SA43-06] (ET1601-28)
Immunogen
Recombinant protein within human jnk1 aa 1-423 / 427.
Host
Rabbit
Positive Control
HeLa cell lysate, HEK-293 cell lysate, Jurkat cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, NIH/3T3, human breast cancer tissue, human brain tissue, mouse brain tissue, rat brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SA43-06
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:50
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IF-Tissue
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1:50
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IHC-P
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1:400-1:1,000
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 48/53 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
JNK1+JNK2+JNK3
SYNONYMS
C Jun kinase 2 antibody;c Jun N terminal kinase 1 antibody;c Jun N terminal kinase 2 antibody;c Jun N terminal kinase 3 antibody;c-Jun N-terminal kinase 1 antibody;JNK 46 antibody;JNK 55 antibody;JNK antibody;JNK-46 antibody;JNK1 antibody;JNK1A2 antibody;JNK2 antibody;JNK21B1/2 antibody;JNK2A antibody;JNK2ALPHA antibody;JNK2B antibody;JNK2BETA antibody;JNK3 alpha protein kinase antibody;JNK3 antibody;JNK3A antibody;Jun kinase antibody;JUN N terminal kinase antibody;MAP kinase 10 antibody;MAP kinase 8 antibody;MAP kinase 9 antibody;MAP kinase p49 3F12 antibody;MAPK 10 antibody;MAPK 8 antibody;MAPK 9 antibody;MAPK10 antibody;mapk8 antibody;MAPK9 antibody;Mitogen activated protein kinase 10 antibody;Mitogen activated protein kinase 8 antibody;Mitogen activated protein kinase 8 isoform JNK1 alpha1 antibody;Mitogen activated protein kinase 8 isoform JNK1 beta2 antibody;Mitogen activated protein kinase 9 antibody;Mitogen-activated protein kinase 8 antibody;MK08_HUMAN antibody;p493F12 antibody;p54a antibody;p54aSAPK antibody;p54bSAPK antibody;PRKM10 antibody;PRKM8 antibody;PRKM9 antibody;SAPK antibody;SAPK(beta) antibody;SAPK1 antibody;SAPK1a antibody;SAPK1b antibody;SAPK1c antibody;Stress activated protein kinase 1 antibody;Stress activated protein kinase 1a antibody;Stress activated protein kinase 1b antibody;Stress activated protein kinase 1c antibody;Stress activated protein kinase beta antibody;Stress activated protein kinase JNK1 antibody;Stress activated protein kinase JNK2 antibody;Stress activated protein kinase JNK3 antibody;Stress-activated protein kinase 1c antibody;Stress-activated protein kinase JNK1 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
POST-TRANSLATIONAL MODIFICATION
Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Phosphorylated by TAOK2. May be phosphorylated at Thr-183 and Tyr-185 by MAP3K1/MEKK1. Phosphorylated form is more concentrated at synapses than none-phosphorylated (By similarity).
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Membrane, Mitochondrion
FUNCTION
The c-Jun N-terminal kinases consist of ten isoforms derived from three genes: JNK1 (four isoforms), JNK2 (four isoforms) and JNK3 (two isoforms). Each gene is expressed as either 46 kDa or 55 kDa protein kinases, depending upon how the 3' coding region of the corresponding mRNA is processed. There have been no functional differences documented between the 46 kDa and the 55 kDa isoform, however, a second form of alternative splicing occurs within transcripts of JNK1 and JNK2, yielding JNK1-α, JNK2-α and JNK1-β and JNK2-β. Differences in interactions with protein substrates arise because of the mutually exclusive utilization of two exons within the kinase domain. and JNK2 are found in all cells and tissues. JNK3 is found mainly in the brain, but is also found in the heart and the testes.
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