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PRODUCT CODE: ET1702-63

Jagged1 Recombinant Rabbit Monoclonal Antibody [JF96-06] (ET1702-63)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Jagged1 on A431 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-63, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of Jagged1 on A431 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-63, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Jagged1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Jagged1 antibody. Counter stained with hematoxylin.
Western blot analysis of Jagged1 on A431 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-63, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Jagged1 Recombinant Rabbit Monoclonal Antibody [JF96-06] (ET1702-63)

Immunogen

Synthetic peptide within human jagged1 aa 1170-1218.

Host

Rabbit

Positive Control

Human kidney tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF96-06

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

134 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

P78504

PROTEIN NAME

Jagged1

SYNONYMS

AGS antibody; AHD antibody; AWS antibody; CD 339 antibody; CD339 antibody; CD339 antigen antibody; Headturner antibody; hJ1 antibody; Htu antibody; Jag 1 antibody; Jag1 antibody; JAG1_HUMAN antibody; Jagged 1 antibody; Jagged1 (Alagille syndrome) antibody; Jagged1 antibody; JAGL1 antibody; MGC104644 antibody; OTTHUMP00000030278 antibody; Protein jagged-1 antibody; Ser 1 antibody; Ser1 antibody; Serrate 1 antibody; Slalom antibody

TISSUE SPECIFICITY

Widely expressed in adult and fetal tissues. In cervix epithelium expressed in undifferentiated subcolumnar reserve cells and squamous metaplasia. Expression is up-regulated in cervical squamous cell carcinoma. Expressed in bone marrow cell line HS-27a which supports the long-term maintenance of immature progenitor cells.

DEVELOPMENTAL STAGE

Expressed in 32-52 days embryos in the distal cardiac outflow tract and pulmonary artery, major arteries, portal vein, optic vesicle, otocyst, branchial arches, metanephros, pancreas, mesocardium, around the major bronchial branches, and in the neural tube.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

The LIN-12/Notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. Ligands for Notch include Jagged1, Jagged2 and Delta. Jagged is a membrane protein and can activate Notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes. It is involved in mammalian cardiovascular development and in cell-fate decisions during hematopoiesis. Jagged is expressed in adult and fetal tissues, and expression is upregulated in cervical squamous cell carcinoma. Familial Tetralogy of Fallot, the most common form of complex congenital heart disease, is caused by a mutation in the Jagged1 gene.