IDH1 Mouse Monoclonal Antibody [A11-B4] (EM40705)
Catalog# EM40705
Mouse Monoclonal to IDH1
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WB
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IHC-P
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Human
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Mouse
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Rat
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Monkey
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Zebrafish
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: A431 cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: PC-12 cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 42 kDa
Exposure time: 15 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM40705) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
IDH1 Mouse Monoclonal Antibody [A11-B4] (EM40705)
Immunogen
Synthetic peptide within human idh1 aa 101-150 / 414.
Host
Mouse
Positive Control
HepG2 cell lysate, HeLa cell lysate, HEK-293 cell lysate, A431 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate, COS-1 cell lysate, human colon carcinoma tissue, human breast tissue, human kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A11-B4
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:100-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 47 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
IDH1
SYNONYMS
Cytosolic NADP isocitrate dehydrogenase antibody;Cytosolic NADP-isocitrate dehydrogenase antibody;Epididymis luminal protein 216 antibody;Epididymis secretory protein Li 26 antibody;HEL-216 antibody;HEL-S-26 antibody;ICDH antibody;IDCD antibody;IDH antibody;IDH1 antibody;IDHC_HUMAN antibody;IDP antibody;IDPC antibody;Isocitrate dehydrogenase [NADP] cytoplasmic antibody;Isocitrate dehydrogenase 1 (NADP+) soluble antibody;NADP dependent isocitrate dehydrogenase cytosolic antibody;NADP dependent isocitrate dehydrogenase peroxisomal antibody;NADP(+)-specific ICDH antibody;Oxalosuccinate decarboxylase antibody;PICD antibody
SEQUENCE SIMILARITIES
Belongs to the isocitrate and isopropylmalate dehydrogenases family.
POST-TRANSLATIONAL MODIFICATION
Acetylation at Lys-374 dramatically reduces catalytic activity.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Isocitrate dehydrogenase (IDH) is an enzyme that catalyzes the oxidative decarboxylation of isocitrate, producing alpha-ketoglutarate (α-ketoglutarate) and CO2. In humans, IDH exists in three isoforms: IDH3 catalyzes the third step of the citric acid cycle while converting NAD+ to NADH in the mitochondria. The isoforms IDH1 and IDH2 catalyze the same reaction outside the context of the citric acid cycle and use NADP+ as a cofactor instead of NAD+. They localize to the cytosol as well as the mitochondrion and peroxisome. Mutations in IDH1 are also implicated in cancer. Originally mutations in IDH1 were detected in an integrated genomic analysis of human glioblastoma multiforme. In addition to being mutated in diffuse gliomas, IDH1 has also been shown to harbor mutations in human acute myeloid leukemia (AML).
CITATIONS
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He Jing,et al
RNAi screens identify HES4 as a regulator of redox balance supporting pyrimidine synthesis and tumor growth
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WB
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Human
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Citation
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Wang Rui,et al
Screening for ferroptosis genes related to endometrial carcinoma and predicting of targeted drugs based on bioinformatics
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IHC
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Human
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Citation
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Dr Xiaopeng Shen; Dr Guoping Zhu
Wild-type IDH1 affects cell migration by modulating the PI3K/AKT/mTOR pathway in primary glioblastoma cells
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WB
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Human
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Citation
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