IRS-2 Rabbit Polyclonal Antibody (HA500415)
Catalog# HA500415
Rabbit Polyclonal to IRS-2
Tested for
-
WB
-
IHC-P
Reacts with
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Human
-
Rat
-
Mouse
Western blot analysis of IRS-2 on different lysates with Rabbit anti-IRS-2 antibody (HA500415) at 1/1,000 dilution.
Lane 1: 293 cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: A375 cell lysate
Lane 4: Hela cell lysate
Lane 5: PC-12 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 137 kDa
Observed band size: 150/200 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500415) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: 293 cell lysate
Lane 2: SH-SY5Y cell lysate
Lane 3: A375 cell lysate
Lane 4: Hela cell lysate
Lane 5: PC-12 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 137 kDa
Observed band size: 150/200 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500415) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.