IRG1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH07-31] (HA751152)
Catalog# HA751152
Rabbit Monoclonal to IRG1
Tested for
-
WB
-
IF-Cell
Reacts with
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of IRG1 on different lysates with Rabbit anti-IRG1 antibody (HA751152) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate
Lane 3: J774A.1 cell lysate
Lane 4: J774A.1 treated with 1μg/mL LPS for 24 hours cell lysate
Lane 5: mBMMC cell lysate
Lane 6: mBMMC treated with 0.1μg/mL LPS and 10ng/mL IFN-gamma for 24 hours cell lysate
Lane 7: mBMSC cell lysate
Lane 8: mBMSC treated with 40ng/mL MCSF for 7 days then add 100ng/mL LPS for 48 hours cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751152) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of IRG1 on different lysates with Rabbit anti-IRG1 antibody (HA751152) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 treated with 0.1μg/mL LPS for 6 hours cell lysate
Lane 3: J774A.1 cell lysate
Lane 4: J774A.1 treated with 1μg/mL LPS for 24 hours cell lysate
Lane 5: mBMMC cell lysate
Lane 6: mBMMC treated with 0.1μg/mL LPS and 10ng/mL IFN-gamma for 24 hours cell lysate
Lane 7: mBMSC cell lysate
Lane 8: mBMSC treated with 40ng/mL MCSF for 7 days then add 100ng/mL LPS for 48 hours cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751152) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.