IRF9 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH08-20] (HA751211)
Catalog# HA751211
Rabbit Monoclonal to IRF9
Tested for
-
WB
-
IF-Cell
-
IP
-
ChIP
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Cell treatment (CT)
Western blot analysis of IRF9 on different lysates with Rabbit anti-IRF9 antibody (HA751211) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 10ng/mL IFN-α1 for 16 hours cell lysate
Lane 3: Daudi cell lysate
Lane 4: Daudi treated with 10ng/mL IFN-α1 for 16 hours cell lysate
Lane 5: Jurkat cell lysate
Lane 6: Jurkat treated with 100nM IFN-α1 for 24 hours cell lysate
Lane 7: THP-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 48 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751211) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of IRF9 on different lysates with Rabbit anti-IRF9 antibody (HA751211) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 10ng/mL IFN-α1 for 16 hours cell lysate
Lane 3: Daudi cell lysate
Lane 4: Daudi treated with 10ng/mL IFN-α1 for 16 hours cell lysate
Lane 5: Jurkat cell lysate
Lane 6: Jurkat treated with 100nM IFN-α1 for 24 hours cell lysate
Lane 7: THP-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 44 kDa
Observed band size: 48 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751211) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.